Efficacy of green tea extract on PC3 prostate cancer cells through upregulation of miR-195 expression and suppression of epithelial to mesenchymal transition  

作　　者：Fatemeh Safari Katayoun Dadeh Amirfard 

机构地区：[1]Department of Biology,Faculty of Science,University of Guilan,Rasht 4193833697,Iran [2]Department of Microbiology,North Tehran Branch,Islamic Azad University,Tehran 1651153511,Iran

出　　处：《Journal of Traditional Chinese Medicine》2022年第5期681-686,共6页中医杂志（英文版）

摘　　要：OBJECTIVE: To evaluate anticancer efficacy of green tea extract(GTE) on PC3 prostate cancer cells. METHODS: By using quantitative real-time polymerase chain reaction(qRT-PCR) and Western blot methods, the expression of miR-195 and the epithelial to mesenchymal transition(EMT) markers such as E-cadherin and vimentin was analyzed. RESULTS: Based on the results of 2D and 3D cell culture models, the inhibition of EMT and up regulation of miR-195 expression were detected. CONCLUSIONS: Our findings will be helpful to design anti-tumor regimens with natural product original, and more studies will be required to identify the related mechanisms involving anticancer activities of green tea via miRNAs.

关 键 词：Green tea extract PC3 cancer cells epithelial-mesenchymal transition miR-195 expression 

分 类 号：R285[医药卫生—中药学]