Efficacy of Qingre Huashi decoction(清热化湿方) on infection of Helicobacter pylori:inhibiting adhesion,antioxidant,and anti-inflammation  

作　　者：HUANG Qiuyue YE Hui SHI Zongming JIA Xiaofen LIN Miaomiao CHU Yingming YU Jing ZHANG Xuezhi 

机构地区：[1]Department of Integrated Traditional Chinese and Western Medicine,Peking University First Hospital,Beijing 100034,China [2]Institute of Integrated Traditional Chinese and Western Medicine,Peking University,Beijing 100034,China

出　　处：《Journal of Traditional Chinese Medicine》2022年第6期915-921,共7页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China:Research on Mechanisms of Jinghua Weikang Capsule Interving HP Infection and Related Inflammation through Adhersin-ROS/MAPKRNFκB Pathway (No. 81973615);the Beijing Natural Science Foundation:the Effect and Mechanism of Jinghua Weikang Capsule Inhibiting the Adhesion Effect of Helicobacter pylori (No. 7172220)。

摘　　要：OBJECTIVE: To investigate the phytochemical profile of Qingre Huashi decoction( 清 热 化 湿 方, QHD) and evaluate the mechanisms rationalizing the use of QHD against Helicobacter pylori(H. pylori)-associated gastritis. METHODS: QHD is composed of 11 herbs, which was prepared by a fixed Pharmacy and concentrated into clear paste. High-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(HPLC-QTOF/MS) was used to detect the phytochemical profile of QHD. The toxicity of QHD against H. pylori and human gastric epithelial cells was evaluated by the toxicology test and cell counting kit-8 assay. The adhesion model was constructed by incubating H. pylori and gastric mucosal epithelial cells for 2 h. The urease assay was used to examine the antiadhesion effects of QHD, and gene expression of adhesins was evaluated by quantitative polymerase chain reaction. The antioxidant activity was assessed by DCFHDA labeling. To evaluate the anti-inflammatory effect, the levels of pro-inflammatory cytokines in the culture supernatant were detected by enzyme-linked immunosorbent assay. RESULTS: HPLC-QTOF/MS profiling indicated the presence of primary compounds 1-20 in QHD. Drug concentration was determined as 1, 2, and 5 mg/m L by the toxic concentration of QHD against H. pylori and human gastric epithelial cells. QHD prevented H. pylori adhesion to the human gastric epithelial cells and reduced levels of reactive oxygen species. QHD also reduced the level of interleukin-8 and other proinflammatory cytokines that were upregulated by H. pylori infection. CONCLUSION: QHD could inhibit H. pylori adhesion, and exert antioxidant and anti-inflammatory effects in vitro.

关 键 词：Helicobacter pylori bacterial adhesion oxidative stress inflammation Qingre Huashi decoction 

分 类 号：R285[医药卫生—中药学]