一株Triton X-100降解菌的分离鉴定及降解条件优化  

A Strain Degrading Nonionic Surfactant Triton X-100:Screening and Its Optimization of Degradation Conditions

作　　者：王宝任 苏婷婷[1] 王战勇 Wang Baoren;Su Tingting;Wang Zhanyong(School of Petrochemical Engineering,Liaoning Petrochemical University,Fushun Liaoning 113001,China;College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang Liaoning 110866,China)

机构地区：[1]辽宁石油化工大学石油化工学院,辽宁抚顺113001 [2]沈阳农业大学生物科学技术学院,辽宁沈阳110866

出　　处：《辽宁石油化工大学学报》2022年第6期16-20,共5页Journal of Liaoning Petrochemical University

基　　金：辽宁省兴辽英才计划青年拔尖人才项目(XLYC1807034)。

摘　　要：从某化工厂污水处理车间好氧曝气池的活性污泥中,筛选了一株对非离子表面活性剂Triton X-100具有降解能力的菌株ISB5。对菌株的16S rDNA进行测序和比对分析,将菌株ISB5鉴定为假单胞菌属(Pseudomonas sp.)。进一步对菌株ISB5降解Triton X-100的培养条件进行考察,得到了菌株的适宜降解条件:培养温度为30℃,Triton X-100的初始质量浓度为4 g/L,培养时间为28 h,振荡速率为150 r/min,装液量为100.00mL,接种量为1.00%(菌株ISB5与培养基的体积比)。在该条件下,TritonX-100的降解率可达93.0%以上。A strain ISB5 with the ability to degrade the non-ionic surfactant Triton X-100 was screened from the activated sludge in the aerobic aeration tank of a sewage treatment plant in a chemical plant. Through sequencing and comparison analysis of the16S rDNA of the strain, the strain ISB5 was identified as Pseudomonas sp. The culture conditions of strain ISB5 to degrade Triton X-100 were further investigated, and the suitable degradation conditions of the strain were obtained: Culture temperature is 30 ℃,initial concentration of Triton X-100 is 4 g/L, culture time is 28 h, shaking rate is 150 r/min, filling volume is 100.00 mL,inoculation the amount is 1.00%(volume ratio of strain ISB5 to medium). The degradation rate of TritonX-100 by the strain under this condition can reach more than 93.0%.

关 键 词：Triton X-100 生物降解 降解率 

分 类 号：TQ085[化学工程] X703.1[环境科学与工程—环境工程]