严重急性呼吸综合征冠状病毒2受体血管紧张素转换酶2在人肺的表达  

Expression of a receptor of severe acute respiratory syndrome coronavirus 2 angiotensin-converting enzyme 2 in human lung

作　　者：林裕贵 李培群 钟伟娟 解中 李周怡 李程颐 林春秀 张增峰 LIN Yu-gui;LI Pei-qun;ZHONG Wei-juan;XIE Zhong;LI Zhou-yi;LI Cheng-yi;LIN Chun-xiu;ZHANG Zeng-feng(Department of Microbiology,College of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China;Collaborative Innovation Centre for Biomedicine,Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China;State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China)

机构地区：[1]广西医科大学基础医学院微生物学教研室,南宁530021 [2]广西医科大学生物医药协同创新中心,南宁530021 [3]江南大学食品科学与技术国家重点实验室,无锡214122

出　　处：《海军军医大学学报》2022年第11期1251-1256,共6页Academic Journal of Naval Medical University

基　　金：国家自然科学基金(31660041);广西壮族自治区自然科学基金(2018GXNSFAA281300).

摘　　要：目的考察人肺中严重急性呼吸综合征冠状病毒2(SARS-CoV-2)受体血管紧张素转换酶2(ACE2)的表达,探讨ACE2在SARS-CoV-2感染中的意义。方法利用基因型-组织表达(GTEx)数据库分析人体正常组织器官间ACE2的基因转录水平,免疫组织化学检测原代人肺组织中不同解剖部位ACE2的蛋白表达水平。结果人肺中ACE2基因及蛋白表达水平均很低,主要表达于肺泡组织中的少量Ⅱ型肺泡上皮细胞,而在血管内皮组织以及支气管、细支气管、终末细支气管、呼吸性细支气管等各级支气管组织中均未见表达。结论ACE2在人肺中表达水平很低,主要表达于少量Ⅱ型肺泡上皮细胞,表明人肺具有引起SARS-CoV-2感染的ACE2分子基础,SARS-CoV-2在人肺中可能存在其他感染机制。Objective To investigate the expression of angiotensin-converting enzyme 2(ACE2)(a receptor of severe acute respiratory syndrome coronavirus 2[SARS-CoV-2])in human lung and its significance in SARS-CoV-2 infection.Methods Genotype-Tissue Expression(GTEx)database was used to analyze the gene transcription levels of ACE2 in human normal tissues and organs.Immunohistochemistry was used to detect the protein expression levels of ACE2 in different anatomical sites of primary human lung tissues.Results The expression levels of ACE2 gene and protein in human lung were low,which were mainly expressed in a small number of typeⅡalveolar epithelial cells in alveolar tissue,but not in vascular endothelial tissue,bronchial,bronchiole,terminal bronchiole,respiratory bronchiole,or other bronchial tissues.Conclusion The expression of ACE2 in human lung is quite low,and is mainly located in a small number of typeⅡalveolar epithelial cells,indicating that human lung possesses the molecular basis for ACE2 to cause SARS-CoV-2 infection,and SARS-CoV-2 may have other mechanisms of infection in human lung.

关 键 词：血管紧张素转换酶2 肺 严重急性呼吸综合征冠状病毒2 感染 受体 

分 类 号：R373.1[医药卫生—病原生物学]