硒对铁皮石斛组培苗生长、SOD活性及多糖和叶绿素含量的影响  

Effects of Se Application on Growth,SOD Activity and Contents of Polysaccharide and Chlorophyll of Dendrobium officinale Tissue Culture Seedlings

作　　者：李婷[1] 张尚文[1] 杨彬[2] 蒙平[1] 庾韦花[1] 石前 张向军[1] Li Ting;Zhang Shangwen;Yang Bin;Meng Ping;Yu Weihua;Shi Qian;Zhang Xiangjun(Biotechnology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China;Logistics Service Center,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China)

机构地区：[1]广西农业科学院生物技术研究所,广西南宁530007 [2]广西农业科学院后勤服务中心,广西南宁530007

出　　处：《广西林业科学》2022年第6期798-802,共5页Guangxi Forestry Science

基　　金：广西重点研发计划项目(桂科AB17292065);广西特色作物试验站“广西隆安中草药试验站”(桂TS2016002)。

摘　　要：为了解硒对铁皮石斛(Dendrobium officinale)组培苗生长、SOD活性及多糖和叶绿素含量的影响,以铁皮石斛组培苗为材料,研究不同浓度亚硒酸钠溶液对铁皮石斛组培苗长势、SOD活性及多糖和叶绿素含量的影响。结果表明,硒浓度为0.1 mg/L时,铁皮石斛组培苗成活率较高,硒含量在合理范围值内;硒浓度为1 mg/L时,SOD活性、多糖和叶绿素含量最高;硒浓度超过1 mg/L时,硒含量越高,对铁皮石斛组培苗生长、SOD活性及多糖和叶绿素含量的抑制作用越明显。硒浓度小于1 mg/L时,对铁皮石斛组培苗生长最为有利。In order to understand effects of Se on growth,SOD activity and contents of polysaccharide and chlo⁃rophyll of Dendrobium officinale tissue culture seedlings,taking D.officinale tissue culture seedlings as materi⁃als,effects of different concentrations of sodium selenite solutions on growth,SOD activity and contents of poly⁃saccharide and chlorophyll were studied.Results showed that when Se concentration was 0.1 mg/L,survival rate of D.officinale tissue culture seedlings was higher and Se content was within a reasonable range.When Se concentration was 1 mg/L,SOD activity and contents of polysaccharide and chlorophyll were the highest.When Se concentration was higher than 1 mg/L,inhibitory effects of Se on growth,SOD activity and contents of poly⁃saccharide and chlorophyll of D.officinale tissue culture seedlings were more obvious with increase of Se con⁃centration.When Se concentration was less than 1 mg/L,it was the most favorable for growth of D.officinale tis⁃sue culture seedlings.

关 键 词：硒 铁皮石斛组培苗 SOD活性 多糖含量 

分 类 号：S533[农业科学—作物学]