水稻光温敏雄性不育突变体tms3650的鉴定和基因定位  

Identification and Gene Mapping of a New Photo-thermo-sensitive Male Sterile Mutant tms3650 in Rice

作　　者：唐杰 龙湍 吴春瑜 李新鹏 曾翔 吴永忠 黄培劲 TANG Jie;LONG Tuan;WU Chunyu;LI Xinpeng;ZENG Xiang;WU Yongzhong;HUANG Peijin(Hainan Bolian Rice Gene Technology Co.,Ltd.,Haikou 570125,China;College of Tropical Crops,Hainan University,Haikou 570125,China)

机构地区：[1]海南波莲水稻基因科技有限公司,海口510125 [2]海南大学热带作物学院,海口510125

出　　处：《中国水稻科学》2023年第1期45-54,共10页Chinese Journal of Rice Science

基　　金：中国水稻所水稻生物学国家重点实验室开放课题资助项目(20190203)。

摘　　要：【目的】雄性不育是水稻杂种优势利用的基础。为进一步解析其调控机制,对一个雄性不育突变体进行了鉴定。【方法】利用60Co-γ对籼稻品种93-11进行辐射诱变,从其后代中鉴定到一个雄性不育突变体tms3650。将籼稻明恢63作父本同突变体tms3650杂交构建F2和F3群体,采用图位克隆的方法对目的基因进行精细定位。【结果】tms3650突变体其他农艺性状与野生型一致,但花药白绿且瘦小,花粉不能被1%碘-碘化钾溶液染成蓝黑色,穗子包颈,抽穗期延迟。遗传分析表明该突变体表型受一对隐性核基因控制。精细定位结果表明基因位于第3染色体长臂SSR标记RM15927和RM15934之间135.25 kb距离内,且与RM15931标记共分离。陵水冬季南繁鉴定发现,该突变体育性受光温环境影响,说明tms3650突变体的雄性育性在短日低温条件下发生了转育,是一个光温敏雄性不育突变体。【结论】通过将定位位点与已报道的雄性不育基因比较,发现tms3650是一个新的基因位点,暂命名为TMS3650。【Objective】Male sterility is the basis of rice heterosis utilization.To further analyze the regulation mechanism,a male sterility mutant was identified.【Method】The tms3650 mutant was isolated from the mutant library of 60Co-γ-treated indica cultivar 93-11.The indica rice Minghui 63 was crossed with the mutant tms3650 as the male parent to construct F2 and F3 populations,and the target gene was fine-mapped by map-based cloning.【Result】The other agronomic traits of tms3650 were the same as those of the wild type,but the anthers of the mutant were white-green and thin,the pollen could not be stained blue-black by 1%iodine-potassium iodide solution with panicle-enclosure and delayed heading stage.Genetic analysis indicated that the mutant phenotype was controlled by a pair of recessive nuclear genes.The results of fine mapping showed that the gene was located within a distance of 135.25 kb between the SSR markers RM15927 and RM15934 on the long arm of chromosome 3,and co-segregated with the RM15931 marker.The identification of southern breeding in Lingshui in winter revealed that the fertility of mutant was affected by light and temperature,indicating that the tms3650 was a photo-thermo-sensitive male sterile mutant.【Conclusion】By comparing with the reported male sterility genes,the tms3650 was controlled by a new gene locus,TMS3650.

关 键 词：水稻 光温敏雄性不育 基因定位 

分 类 号：S511[农业科学—作物学]