利用分子标记辅助选择培育优良食味、低谷蛋白香粳稻新品系  

Development of New Low Glutelin Content japonica Rice Lines with Good Eating Quality and Fragrance by Molecular Marker-Assisted Selection

作　　者：陈涛[1,2] 赵庆勇 朱镇[1] 赵凌[1] 姚姝[1] 周丽慧[1] 赵春芳[1] 张亚东[1] 王才林[1] CHEN Tao;ZHAO Qingyong;ZHU Zhen;ZHAO Ling;YAO Shu;ZHOU Lihui;ZHAO Chunfang;ZHANG Yadong;WANG Cailin(Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Jiangsu High Quality Rice R&D Center/Nanjing Branch of China National Center for Rice Improvement,Nanjing 210014,China;Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心,南京210014 [2]扬州大学江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009

出　　处：《中国水稻科学》2023年第1期55-65,共11页Chinese Journal of Rice Science

基　　金：江苏省重点研发计划(现代农业)资助项目(BE2022336);现代农业产业技术体系建设专项(CARS-01)。

摘　　要：【目的】选育低谷蛋白新品种是功能性水稻育种的一个重要方向。为满足肾脏病患者对人体健康和稻米品质的需求,迫切需要在育种中加强对功能性和食味品质的协同改良。【方法】以具有低直链淀粉含量基因Wxmp和香味基因fgr的优良食味粳稻品种南粳46与含低谷蛋白基因Lgc1的日本粳稻品种LGC-1杂交、回交,利用与目标基因共分离的分子标记进行跟踪检测并结合田间选择,在BC2F6获得5个新品系。以亲本南粳46和LGC-1为对照,对新品系的农艺、产量、品质性状进行分析。【结果】与LGC-1相比,新品系的谷蛋白含量和可吸收蛋白含量相当,食味品质得到显著提升,且综合性状优良,表现出较高的产量潜力,适宜在江苏不同稻区进行种植。【结论】分子标记辅助选择作为一种快速、准确、有效的方法,与常规育种技术紧密结合,能够显著提高优质、高产、低谷蛋白水稻品种的选育效率。【Objective】Developing new low glutelin content varieties is an important direction of functional rice breeding.It is necessary to strengthen the synchronized improvement of rice functionality and eating quality in breeding to meet the needs of nephropathic patients for rice quality and physical health.【Method】The good eating quality japonica rice variety Nanjing 46 with the low amylose content gene Wxmp and fragrance gene fgr was used as the parent to cross and backcross with the variety LGC-1 from Japan containing the Lgc1 gene.The molecular markers co-isolated with target genes were utilized for genotypic detection in combination with field selection and five new lines were obtained in BC2F6 generation.Taking parents Nanjing 46 and LGC-1 as control,the agronomic,yield and quality traits of these lines were analyzed.【Result】Compared with LGC-1,these lines has similar glutelin content and absorbable protein content,improved eating quality,excellent comprehensive characters and high yield potential.They are suitable to grow in different regions of Jiangsu Province.【Conclusion】As a rapid,accurate and effective method,the molecular marker-assisted selection,in combination with conventional breeding technology,can significantly improve the breeding efficiency of the low gluten rice varieties with good quality and high yield.

关 键 词：粳稻 低谷蛋白含量 优良食味 香味 分子标记辅助选择 

分 类 号：S511.22[农业科学—作物学]