利用激光在微流控芯片中实现细胞分选的研究  

Research of Cell Sorting in Microfluidic Chip by Laser

作　　者：仲小祥 冒卫星[1] 张华丽[1] ZHONG Xiao-xiang;MAO Wei-xing;ZHANG Hua-li(School of Mechanical Engineering,Nantong University,Jiangsu Nantong 226019,China)

机构地区：[1]南通大学机械工程学院,江苏南通226019

出　　处：《机械设计与制造》2023年第1期9-12,共4页Machinery Design & Manufacture

基　　金：江苏省自然科学基金资助项目(BK20150406);江苏省高校优势学科建设工程资助项目(苏财政2018-192号)。

摘　　要：光学操纵由于其无机械损伤的特点在生物医学微芯片中的应用较为广泛,可实现细胞培养、捕获、分选和检测等功能。采用COMSOL Multiphysics仿真分析软件,利用光学操纵,在微通道结构中,模拟细胞群随鞘流的流动并分析细胞受激光力后的运动过程。选用聚苯乙烯微球进行鞘流实验,在100mw激光器的作用下,观察粒子在微流控芯片中的运动情况。结果表明,细胞在鞘流中的偏转受其物理尺寸和激光功率的影响。同时聚苯乙烯实验结果与模拟实验结果相符。根据模拟结果,可以确定用于操纵不同直径细胞的激光功率大小。利用COMSOL Multiphysics建立的细胞操纵模型可进行集成光流控细胞分选,并为光流控细胞分选实验提供参考。There are a variety of applications of optical manipulation in a microchannel,where single cells can be cultured,captured,sorting and detected for its benefits of no mechanical damage.We present a COMSOL Multiphysics simulation to monitor the sheaths flows in a microchannel,for liquids with different cells.With the laser operator,the dynamic movement of cells is investigated from the combination of flowing channel construction and the laser force.In results,the deflection of cells or microparticles in the sheath flow is affected by its diameters and the laser power.This simulation can be extended to determine the laser forces used to manipulate different cells in diameter.The sheath flow experiment of the polystyrene microsphere was carried out,and the trajectory of the microsphere was consistent with the simulation under the action of 100mw laser Because of prediction,the manipulation model using COMSOL Multiphysics can be used as potential integrated optofluidic cell sorting and paves the way for a broad range of simulation applications in biomolecular interactions.

关 键 词：光流控技术 微流控芯片 单细胞操纵 细胞分选 

分 类 号：TH16[机械工程—机械制造及自动化] Q631[生物学—生物物理学] TN24[电子电信—物理电子学] O43[机械工程—光学工程]