无瓣海桑叶斑病及其病原菌的生物学特性研究  

Study on Identification and Biological Characteristics of Leaf Spot of Sonneratia apetala

作　　者：黄华 冯金艳 陆建康 张良军 刘幸儿 田龙艳 HUANG Hua;FENG Jinyan;LU Jiankang;ZHANG Liangjun;LIU Xinger;TIAN Longyan(Forest Pest Control and Quarantine Station,Fengkai County Forestry Bureau,Zhaoqing,Guangdong 526500,China;Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China)

机构地区：[1]肇庆市封开县林业局森林病虫害防治检疫站,广东肇庆526500 [2]广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东广州510520

出　　处：《林业与环境科学》2022年第5期176-180,共5页Forestry and Environmental Science

基　　金：广东省重点领域研发计划项目(2020B020214001);广东省基础与应用基础研究基金项目(2019A1515110596)。

摘　　要：研究以无瓣海桑Sonneratia apetala叶部病组织上分离的菌株WBHS-YB-2A为试材,依据柯赫氏法则测定了其致病性,同时开展了该菌的形态学和生物学特性研究。结果表明:菌株WBHS-YB-2A为无瓣海桑叶斑病的病原菌,该病原菌隶属于拟盘多毛孢属;该菌能利用多种碳源和氮源,其最佳碳源为葡萄糖,最佳氮源为蛋白胨,最适培养温度为25℃,最适pH为6.0;在缺氮、缺碳条件下,菌丝生长缓慢、气生菌丝稀疏,生长势较差。研究明确了无瓣海桑叶斑病为无瓣海桑的一种新病害,其病原菌为拟盘多毛孢属一新种Pestalotiopsis sonneratiae。研究结果丰富了无瓣海桑的病害目录。In this study,we isolated a fungal strain(WBHS-YB-2A)from the leaf spot of Sonneratia apetala.We determined its pathogenicity according to Koch’s rule,and studied its morphological and biological characteristics.The results showed that WBHS-YB-2A were pathogenic fungus of leaf spot disease of S.apetala,which was belonging to the genus of Pestalotiopsis.It used a variety of carbon and nitrogen sources,which optimum carbon and nitrogen sources were glucose and peptone.Under the condition of nitrogen and carbon deficiency,its mycelia grew slowly and sparsely.The optimal temperature and pH for cultivation was 25℃and 6.0,respectively.This research clarified that the leaf spot of S.apetala was a new pathogen,which enriched the disease catalogue.It would provide a basis for the prevention and control the disease of mangrove forests.

关 键 词：Pestalotiopsis sonneratiae 无瓣海桑叶斑病 生物学特性 

分 类 号：S763.1[农业科学—森林保护学]