采用手性固定相高效液相色谱法拆分D-(+)-二苯甲酰酒石酸无水物的异构体  

Resolution of D-(+)-Dibenzoyl Tartaric Acid Anhydrous Isomers by Chiral Stationary Phase HPLC

作　　者：孙琳 周君安 曹秀芝 魏娜 Sun Lin;Zhou Jun′an;Cao Xiuzhi;Wei Na(Jiangsu Hansoh Pharmaceutical Co.,Ltd.,Lianyungang 222000,China)

机构地区：[1]江苏豪森药业集团有限公司,江苏连云港222000

出　　处：《山东化工》2022年第23期169-171,共3页Shandong Chemical Industry

摘　　要：目的:建立了采用高效液相色谱法拆分D-(+)-二苯甲酰酒石酸无水物异构体的方法。方法:采用高效液相色谱法,色谱柱(CHIRALPAK AD-H,4.6 mm×250 mm,5μm);以正己烷-无水乙醇-三氟乙酸(500∶500∶1,体积比)为流动相,等度洗脱10 min;流速为0.8 mL/min;检测波长为230 nm;柱温为35℃;进样体积为10μL。结果:D-(+)-二苯甲酰酒石酸无水物及其异构体在此条件下分离度为6.0,且峰面积与浓度在一定的范围内均呈良好的线性关系。结论:方法验证的结果证明该方法可用于D-(+)-二苯甲酰酒石酸无水物及其异构体的分离,可以准确地检测D-(+)-二苯甲酰酒石酸无水物中异构体的含量。Objective:To establish a method for resolution of D-(+)-dibenzoyl tartaric acid anhydrous isomers.Methods:The D-(+)-dibenzoyl tartaric acid anhydrous isomers was determined by HPLC with CHIRALPAK AD-H(250 mm×4.6 mm,5μm)column;the mobile phase was in the phase of hexane-ethanol-trifluoroacetic acid(500∶500∶1,volume ratio).The flow rate was 0.8 mL/min;detection wavelength was 230 nm;column temperature was 35℃.Results:The results of method validation proved that this method can be used for the separation of D-(+)-dibenzoyl tartaric acid anhydrous and its isomers,can accurately detect the content of isomers in D-(+)-dibenzoyl tartaric acid anhydrous.

关 键 词：D-(+)-二苯甲酰酒石酸无水物 高效液相色谱法 异构体分离 手性固定相 

分 类 号：O657.7[理学—分析化学]