加味星蒌承气颗粒对大鼠局灶性脑缺血的保护作用研究  

Protective Effect of Jiawei Xinglou Chengqi(加味星蒌承气)Granules on Focal Cerebral Ischemia

作　　者：任桂林 蒲清荣 杜仕静 谭正怀[2] 黄锐 董丽 刘平 赵剑 杨思进 Ren Guilin;Pu Qingrong;Du Shijing;Tan Zhenghuai;Huang Rui;Dong Li;Liu Ping;Zhao Jian;Yang Sijin(The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University,Luzhou 646000;Sichuan Academy of Chinese Meteria Medica,Chengdu 610000)

机构地区：[1]西南医科大学附属中医医院,泸州646000 [2]四川省中医药科学院,成都610000

出　　处：《中药药理与临床》2022年第5期69-72,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：四川省中医药管理局重点项目(编号:2021ZD015);四川省中医药管理局项目(编号:2022CP3561)。

摘　　要：目的:评价加味星蒌承气颗粒对大鼠局灶性脑缺血的保护作用。方法:以加味星蒌承气颗粒为研究对象,采用大脑中动脉闭塞(MCAO)致局灶性脑缺血大鼠模型、小鼠断头致全脑缺氧模型,检测神经功能、脑组织生化以及病理组织学等相关指标。结果:与假手术对照组比较,模型对照组大鼠体质量减轻,脑组织出现较大面积缺血、坏死、炎细胞浸润、含水率增加、神经行为学评分显著增加,血清SOD活力明显降低(P<0.05或P<0.01);与模型对照组相比,加味星蒌承气颗粒32 g/kg大鼠的体质量增加;加味星蒌承气颗粒16、32 g/kg能明显降低MCAO大鼠脑梗死面积,减轻脑组织水肿,升高血清中SOD活力,改善MCAO模型大鼠神经评分,改善脑细胞肿胀坏死程度,炎细胞浸润等组织学评分(P<0.05或P<0.01);与假手术对照组比较,加味星蒌承气颗粒20 g/kg能明显增加小鼠张口呼吸次数,显著提高小鼠脑中GSH的含量(P<0.05)。结论:加味星蒌承气颗粒对脑中风具有较好的防治作用,可能与其具有抗氧化、神经保护作用有关。Objevtive:To evaluate the protective effect of Jiawei Xinglou Chengqi Granules on focal cerebral ischemia.Methods:Taking Jiawei Xinglou Chengqi Granules as the research object,the rat model of focal cerebral ischemia was established by middle cerebral artery occlusion(MCAO),and the mouse model of whole brain hypoxia was established by decapitation.The related indexes such as neurological function,brain tissue biochemistry,and histopathology were detected.Results:As compared with the sham control group,the body weight was reduced in the model control group,large areas of ischemia and necrosis,inflammatory cell infiltration,increased brain water content,and increased neurobehavioral score were observed in the brain tissue,while the serum superoxide dismutase(SOD)activity was decreased(P<0.05).Compared with the model control group,the 32.0 g/kg Jiawei Xinglou Chengqi Granule group increased the body weight.Both the 16.0 g/kg and 32.0 g/kg Jiawei Xinglou Chengqi Granule groups reduced the area of cerebral infarction,alleviated brain edema,increased the serum SOD activity,and improved the neurological score of the MCAO-induced rat model,the degree of brain cell swelling and necrosis,and the histological scores of inflammatory cell infiltration(P<0.05 or P<0.01).Compared with the sham control group,the 20 g/kg Jiawei Xinglou Chengqi Granule group significantly increased the number of mouth-opening and the content of glutathione(GSH)in the brain tissue(P<0.05).Conclusion:Jiawei Xinglou Chengqi Granules have a good preventive and therapeutic effect on cerebral apoplexy,which may be related to their antioxidant and neuroprotective effects.

关 键 词：加味星蒌承气颗粒 局灶性脑缺血 脑梗死 神经保护 抗氧化 

分 类 号：R285.5[医药卫生—中药学]