2018-2020年云南省边境地区猪群PRRSV抗体检测与分析  

Antibody Detection and Analysis of Porcine Reproductive and Respiratory Syndrome Virus in Border Areas of Yunnan Province from 2018 to 2020

作　　者：谢佳芮 寇美玲 李乐[1] 苗海生[1] XIE Jia-rui;KOU Mei-ling;LI Le;MIAO Hai-sheng(Yunnan Tropical and Subtropical Animal Virus Disease Laboratory,Yunnan Animal Science and Veterinary Institute,Kunming Yunnan 650224,China)

机构地区：[1]云南省畜牧兽医科学院,云南省热带亚热带动物病毒病重点实验室,云南昆明650224

出　　处：《畜牧兽医杂志》2023年第1期118-123,共6页Journal of Animal Science and Veterinary Medicine

基　　金：云南省技术创新人才培养对象(2018HB076);国家重点研发计划(2017YFC1200502)。

摘　　要：为了解和对比云南边境地区和其他地区2018-2020年猪繁殖与呼吸综合征(PRRS)免疫保护水平,疫情发生风险,2018-2020年在云南与缅甸、老挝、越南接壤的14个边境县和其他地区的规模场、散养户采集猪血清,应用猪繁殖与呼吸综合征病毒(PRRSV)ELISA抗体检测方法进行病毒抗体检测。检测结果显示:2900份猪血清样品中,阳性样品2179份,群体阳性率为75.14%(2179/2900),S/P平均值为1.0510±0.7484,变异系数为70.77%。各县PRRSV抗体阳性率在50%~90%之间,并且不同地区存在差异。抗体阳性率最高为孟连91.33%(274/300)。不同阶段猪群PRRSV平均抗体水平存在差异,哺乳仔猪抗体阳性率最低为48.14%(297/617),育肥猪抗体阳性率最高87.30%(949/1087)。入境猪群和境内猪群抗体阳性率差异显著(P<0.01),养殖场,屠宰场,交易市场猪群PRRSV抗体阳性率要高于自然散养户。本研究结果表明:猪群PRRSV抗体水平在云南边境地区整齐度不高,且地区间存在差异,入境猪群有传播疫情的风险,因此可依据边境地区的养殖特点,制定科学合理的免疫措施及时控制疫病流行,同时继续加强云南省边境地区的PRRSV防控工作。To understand and compare the level of immune protection and epidemic risk of porcine reproductive and respiratory syndrome(PRRS)between Yunnan border areas and other areas in 2018-2020.During 2018-2020,we collected pig serum from large-scale farms and free-range farms in 14 border counties of Yunnan bordering Myanmar,Laos and Vietnam and other areas.Porcine Reproductive and Respiratory syndrome virus(PRRSV)ELISA was used to detect PRRSV antibody.The results showed that 2,179 of 2,900 pig serum samples were positive,with a population positive rate of 75.14%(2179/2900).The mean value of S/P was 1.0510±0.7484,and the coefficient of variation was 70.77%.The positive rate of PRRSV antibody was between 50%and 90%in all counties,and there were differences in different regions.The highest antibody positive rate was 91.33%(274/300)of Menglian.The average antibody level of PRRSV was different in different pig groups,with the lowest positive rate of 48.14%(297/617)in Suckling piglets and the highest positive rate of87.30%(949/1087)in finishing pigs.The antibody positive rate of inbound and domestic pigs was significantly different(P<0.01).The positive rate of PRRSV antibody in pig farms,slaughterhouses and trading markets was higher than that in natural free-range farms.The results of this study indicated that the level of PRRSV antibody in pigs in the border areas of Yunnan was not high,and there were differences between regions,and the imported pigs were at risk of spreading the epidemic.Therefore,according to the characteristics of breeding in border areas,scientific and reasonable immunization measures can be formulated to timely control the epidemic,and at the same time,the prevention and control of PRRSV in border areas of Yunnan Province can be strengthened.

关 键 词：边境地区 抗体检测 入境猪群 

分 类 号：S851.43[农业科学—预防兽医学]