宫炎平片中没食子酸、氯化两面针碱、阿魏酸含量测定  

Determination of Gallic Acid,Anaphylline Chloride and Ferulic Acid in Gongyanping Tablets

作　　者：古炳明 邬伟魁 刘佳 Gu Bingming;Wu Weikui;Liu Jia(Meizhou Food and Drug Administration,Meizhou 51401l,China)

机构地区：[1]梅州市食品药品监督检验所,广东梅州1514011

出　　处：《亚太传统医药》2022年第12期68-71,共4页Asia-Pacific Traditional Medicine

基　　金：广东省药品监督管理局科技创新立项项目(2021TDB09);广东省药品监督管理局市县药品监管综合改革创新立项项目(ZG-DS-2022019)。

摘　　要：目的:探讨宫炎平片中没食子酸、氯化两面针碱、阿魏酸的含量。方法:液相色谱条件:SUPELCO Discovery C18色谱柱(25cm×4.6mm,5μm),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,进行梯度洗脱(0~12min:3%A,12~70min:3%A~40%A);体积流量:1.0mL/min;柱温:30℃;检测波长:没食子酸和氯化两面针碱271nm,阿魏酸323nm;进样量:10μL。结果:没食子酸、阿魏酸和氯化两面针碱线性范围峰面积与浓度呈现良好的线性关系;没食子酸、阿魏酸和氯化两面针碱的平均加样回收率分别为99.58%(RSD=0.65%)、98.57%(RSD=0.81%)和99.08%(RSD=1.33%)。结论:该方法操作简便,重现性较好,适用于宫炎平片中没食子酸、阿魏酸、氯化两面针碱的含量测定。Objective:To investigate the contents of gallic acid,anaphylline chloride and ferulic acid in Gongyanping tablets.Methods:Liquid chromatography conditions:SUPELCO Discovery C18Column(25cm×4.6 mm,5μm),with acetonitrile(A)-0.1% phosphoric acid aqueous solution(B)as mobile phase,gradient elution(0~12min:3%A,12~70min:3%A~40%A).Volume flow:1.0mL/min;column temperature 30°C;detection wavelength:271nm for gallic acid and anaphylline chloride,323nm for ferulic acid;injection volume:10μL.Results:The linear range of gallic acid,ferulic acid and anaphylline chloride showed a good linear relationship between the peak area and the concentration.The mean loading recoveries of gallic acid,ferulic acid and anaphylline chloride were 99.58%(RSD=0.65%),98.57%(RSD=0.81%)and 99.08%(RSD=1.33%),respectively.Conclusion:The method is easy to operate and has good reproducibility,and is suitable for the determination of gallic acid,ferulic acid and anaphylline chloride in Gongyanping tablets.

关 键 词：宫炎平片 高效液相色谱 含量测定 

分 类 号：R284.2[医药卫生—中药学]