雌激素干预的EPCs移植对糖尿病缺血性脑卒中大鼠的治疗作用  

Therapeutic effects of estrogen-intervened EPCs transplantation on diabetic ischemic stroke rats

作　　者：董雅芬[1] 王建[1] 陈业 李姝姝 刘河龙 邱彦 DONG Yafen;WANG Jian;CHEN Ye;LI Shushu;LIU Helong;QIU Yan(Shanghai Pudong New Area People’s Hospital,Shanghai 201200,China)

机构地区：[1]上海市浦东新区人民医院,上海201200

出　　处：《药学实践与服务》2023年第1期40-44,49,共6页Journal of Pharmaceutical Practice and Service

基　　金：浦东新区卫生健康委员会领先人才培养项目(PRWL2020-08);上海市浦东新区人民医院科技项目(E17-04)。

摘　　要：目的探讨雌激素干预的EPCs移植对糖尿病缺血性脑卒中的治疗作用。方法制备大鼠糖尿病缺血性脑卒中模型,24 h后通过尾静脉移植经PKH26标记的糖尿病EPCs和雌激素干预的糖尿病EPCs。移植3 d后测定各组大鼠脑缺血体积、大鼠行为学变化、缺血部位血管新生及EPCs的归巢情况。结果与糖尿病脑缺血大鼠相比,雌激素干预的糖尿病EPCs移植能降低脑缺血体积、改善行为学评分和缺血部位的血管新生及促进EPCs归巢到损伤部位(P<0.05)。结论雌激素干预的糖尿病EPCs移植,通过促进EPCs归巢和血管新生对糖尿病缺血性脑卒中有较好的治疗作用。Objective To explore the therapeutic effects of estrogen-intervened endothelial progenitor cells(EPCs)transplantation on diabetic ischemic stroke rats.Methods PKH26-labeled diabetic EPCs and estrogen-intervened diabetic EPCs were injected into rats via the tail vein 24 h after cerebral ischemia.Cerebral ischemic volume,behavioral changes,ischemic site vascularization and homing of EPCs were measured 3 d after EPCs injection.Results Compared with diabetic ischemic rats,estrogen-intervened EPCs transplantation had reduced infarct volumes,improved behavioral scores and ischemic site revascularization and promoted homing of EPCs to sites of injury(P<0.05).Conclusion Estrogen-intervened EPCs transplantation had a better therapeutic effect on diabetic ischemic stroke by promoting EPCs homing to injury site and EPCs-medicated neovascularization.

关 键 词：雌激素 内皮祖细胞移植 糖尿病缺血性脑卒中 血管新生 归巢 

分 类 号：R587.2[医药卫生—内分泌] R743.3[医药卫生—内科学]