正常及脾虚湿阻型单纯性肥胖大鼠脾组织蛋白组学差异分析  

Variance Analysis on the Proteomics of Spleen Tissue in Normal and Simple Obesity Rats with Spleen Deficiency and Dampness Resistance

作　　者：刘芳芳 李琳荣 王平 张耀 师新宇[2] 李娟 张赞 陶功定 郭晓峰 李俊莲(指导) LIU Fangfang;LI Linrong;WANG Ping;ZHANG Yao;SHI Xinyu;LI Juan;ZHANG Zan;TAO Gongding;GUO Xiaofeng;LI Junlian(School of Basic Medicine,Shanxi University of Traditional Chinese Medicine,Shanxi Province,Jinzhong 030619,China;Office of the Second Hospital of Shanxi Medical University,Shanxi Province,Taiyuan 030001,China)

机构地区：[1]山西中医药大学基础医学院,山西晋中030619 [2]山西医科大学第二医院办公室,山西太原030001

出　　处：《光明中医》2022年第23期4277-4280,共4页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：山西省自然科学基金青年项目(No.201801D221435);山西省高等学校创新项目(No.2019L0719);山西中医药大学科技创新项目(No.2019PY035)。

摘　　要：目的通过比较正常及脾虚湿阻型单纯性肥胖大鼠脾组织蛋白质谱,筛选和鉴定与单纯性肥胖大鼠脾虚湿阻证有关的疾病特异性蛋白,探寻单纯性肥胖大鼠脾虚湿阻证生物标志物。方法选取SD大鼠40只随机分为对照组10只、单纯性肥胖大鼠组30只。造模采用高脂饲料喂养,造模成功后无菌摘除脾脏,取出脾组织10 mg/只,进行酶切、双向凝胶电泳分析,质谱鉴定蛋白质,分析差异蛋白质意义。结果通过比较正常及脾虚湿阻型单纯性肥胖大鼠脾组织差异蛋白质,发现3个有意义的差异蛋白质:巨噬细胞中穹窿主体蛋白(Major Vault Protein,MVP)、蛋白质二硫化物异构酶A3前体(PDIA3)、60-kDa热休克蛋白(HSP60,也称为HSPD1)。结论正常及脾虚湿阻型单纯性肥胖大鼠脾组织蛋白质存在差异表达。这些差异蛋白质可能是与脾虚湿阻证相关的疾病特异性蛋白,并有可能成为诊断脾虚湿阻证的分子标志物。Objective To screen and identify the disease-specific proteins related to spleen deficiency and dampness resistance syndrome of simple obesity rats by comparing the protein mass spectra of normal and simple obesity rats with spleen deficiency and dampness resistance,and to explore biomarkers of spleen deficiency and dampness resistance syndrome in simple obesity rats.Methods 40 SD rats were randomly divided into control group(n=10)and simple obesity group(n=30).The model was fed with high-fat diet.After successful modeling,the spleen was removed by asepsis and every rate was removed 10 mg from the spleen.Enzyme digestion and two-dimensional gel electrophoresis were used to identify proteins and analyze the significance of differential proteins.Results By comparing the differential proteins in spleen tissue of normal and simple obesity rats with spleen deficiency dampness resistance type,three significant differential proteins were found:major vault protein(MVP),protein disulfide isomerase A3 precursor(pdia3),60 k Da heat shock protein(Hsp60,also known as hspd1).Conclusion The protein expression in spleen tissue of normal and simple obesity rats with spleen deficiency and dampness resistance type is different.These differential proteins may be disease-specific proteins related to spleen deficiency dampness syndrome,and may become molecular markers for the diagnosis of spleen deficiency dampness syndrome.

关 键 词：脾虚湿阻证 单纯性肥胖 脾组织 差异蛋白质 实验研究 

分 类 号：R259[医药卫生—中西医结合] R-332[医药卫生—中医内科学]