Application of yeast spores as β-glucan particles  

作　　者：Guoyu Liu Yan Yang Hiroyuki Tachikawa Xiao-Dong Gao Hideki Nakanishi 

机构地区：[1]Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China [2]Department of Applied Biological Chemistry,Graduate School of Agricultural and Life Sciences,University of Tokyo,Tokyo 113-8657,Japan [3]Collaborative Research Institute for Innovative Microbiology,University of Tokyo,Tokyo 113-8657,Japan

出　　处：《Particuology》2022年第12期34-40,共7页颗粒学报（英文版）

基　　金：This work is supported by National First-class Discipline Program of Light Industry Technology and Engineering(LITE2018-015);Project 111(111-2-06);Collaborative Innovation Center of jiangsu Modern Industrial Fermentation,International Joint Research Laboratory for production of therapeutic glycoproteins at Jiangnan University to X.-D.Gao;Fundamental Research Funds for the Central Universities to H.Nakanishi(JUSRP51629B);National Natural Science Foundation of China grants to H.Nakanishi(32071467)and X.-D.Gao(21778023).

摘　　要：β-Glucan exhibits various beneficial biological activities including immunostimulation.This polysaccha-ride is present in the cell wall of microorganisms including the yeast Saccharomyces cerevisiae.Although vegetative yeast cells are used as dietary supplements,β-glucan in the vegetative cell wall is covered with mannan.During nutrient starvation,yeast cells differentiate into dormant cell spores.β-Glucan is also present in the spore wall,but spores have been poorly studied as application tools.The spore wall consists of four layers andβ-glucan forms the third layer from the outside.However,unlike vegeta-tive cells,theβ-glucan layer can be exposed to the cell surface in spores by genetic manipulation.For example,chs3Δmutant spores exhibit such a spore wall configuration.Dectin-1 is the primary receptor forβ-glucan in mammals and,its activation induces phagocytosis and proinflammatory cytokine secre-tions.We found that chs3Δspores are phagocytosed in mammalian cells in a manner dependent on dectin-1 and induce higher levels of TNF-αand IL-6 than vegetative cells.Our results demonstrate that chs3Δspores areβ-glucan particles with immunostimulatory activities.Thus,they would be attractive materials for application in the food industry.

关 键 词：SACCHAROMYCESCEREVISIAE Sporewall Β-GLUCAN DECTIN-1 IMMUNOSTIMULATION 

分 类 号：O629.1[理学—有机化学]