电针“水沟”对大鼠急性脑梗死后远隔部位继发性损伤的影响  

Effect of Electro-acupuncture “Shuigou(DU26)” on Secondary Injury to the Distal Compartment after Acute Cerebral Infarction in Rats

作　　者：李兰竹 莫燕丽 陈书俞 潘嘉欣 徐林新 刘建浩[2] LI Lan-Zhu;MO Yan-Li;CHEN Shu-Yu;PAN Jia-Xin;XU Lin-Xin;LIU Jian-Hao(Graduate School of Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China;Sanya Traditional Chinese Medicine Hospital,Sanya 572000 Hainan,China)

机构地区：[1]广州中医药大学研究生院,广东广州510006 [2]三亚市中医院,海南三亚572000

出　　处：《广州中医药大学学报》2022年第11期2607-2614,共8页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：海南省自然科学基金面上项目(编号:817399);海南省科技厅重点研发项目(编号:ZDYF2022SHFZ129);海南省临床医学研究中心中医康复学资助项目。

摘　　要：【目的】探讨电针水沟穴对急性脑梗死大鼠的治疗作用及机制。【方法】将60只SD大鼠随机分为假手术组、大脑中动脉闭塞(MCAO)组、MCAO+皮层扩散性抑制(CSD)组和电针组,每组再分为造模后6 h、24 h、72 h 3个亚组,每亚组5只。采用改良Zea Longa再灌注法制备大鼠MCAO模型,再采用机械针刺的方式诱导产生单波CSD构建MCAO伴随CSD模型。造模成功后,电针组电针水沟穴,每次30 min。干预结束后,采用Zea Longa评分对大鼠神经行为学进行评估,末端脱氧核苷酸转移酶介导的dUTP缺口末端标记(TUNEL)染色法观察海马远隔CA1区神经细胞凋亡情况,免疫荧光标记法观测海马远隔CA1区单羧酸转运体1(MCT1)/胶质纤维酸性蛋白(GFAP)共表达情况,定磷法检测海马远隔CA1区Na^(+)-K^(+)-ATP酶活性。【结果】(1)与MCAO+CSD组比较,电针组6、24、72 h时相神经功能缺损评分明显降低(P<0.05)。(2)与假手术组比较,其余3组各时相海马远隔CA1区神经细胞凋亡指数均显著升高(P<0.05);与MCAO组比较,MCAO+CSD组各时相神经细胞凋亡指数明显升高(P<0.05);与MCAO+CSD组比较,电针组各时相神经细胞凋亡指数均明显降低(P<0.05)。(3)与假手术组比较,MCAO组、MCAO+CSD组各时相Na^(+)-K^(+)-ATP酶活性均显著降低(P<0.05);与MCAO组比较,MCAO+CSD组6 h、24 h时相Na^(+)-K^(+)-ATP酶活性显著降低(P<0.05);与MCAO+CSD组比较,电针组6 h、24 h时相Na^(+)-K^(+)-ATP酶活性均显著提高(P<0.05)。(4)与假手术组比较,MCAO+CSD组各时相MCT1/GFAP共表达量显著升高(P<0.05),MCAO组、电针组在24 h时相显著升高(P<0.05);与MCAO组比较,MCAO+CSD组各个时相MCT1/GFAP共表达量显著升高(P<0.05),电针组在24 h时相MCT1/GFAP共表达量显著降低(P<0.05);与MCAO+CSD组比较,电针组各个时相MCT1/GFAP共表达量显著降低(P<0.05)。【结论】电针水沟穴可有效改善大鼠急性脑梗死后远隔部位继发性损伤,其机制可能与提高海马远隔区Na^Objective To investigate the therapeutic effect and mechanism of electro-acupuncture at Shuigou(DU26)acupoint in rats with acute cerebral infarction.Methods Sixty SD rats were randomly divided into the sham-operation group,the middle cerebral artery occlusion(MCAO)group,MCAO+cortical spreading depression(CSD)group and electro-acupuncture group,and each group was further divided into 3 subgroups of 5 rats:6 h,24 h and 72 h.A modified Zea Longa reperfusion method was used to prepare the rat MCAO model,and then mechanical acupuncture was used to induce a single wave of CSD to construct the MCAO with CSD model.After successful modeling,the electro-acupuncture group applied electro-acupuncture at the Shuigou acupoint for 30 minutes each time.At the end of the intervention,the neurobehaviour of the rats was assessed using the Zea Longa scoring,terminal deoxynucleotide transferase-mediated dUTP Nick end labeling(TUNEL)staining method was used to observe the apoptosis of neurons in the distant CA1 region of hippocampus,the co-expression of monocarboxylic acid transporter 1(MCT1)/glial fibrillary acidic protein(GFAP)in the distant CA1 region of hippocampus was observed by immunofluorescence labeling method,and the activity of Na^(+)-K^(+)-ATPase in distant CA1 region of hippocampus was detected by fixed phosphorus method.Results(1)Compared with the MCAO+CSD group,the neurofunction deficit score was significantly decreased in the electro-acupuncture group at 6-,24-,72-hour time phases(P<0.05).(2)Compared with the sham-operation group,the apoptotic index of neuronal cells in the distal CA1 region of the hippocampus was significantly increased in the remaining three groups at all time phases(P<0.05);compared with the MCAO group,the apoptosis index of neural cells in each phase of MCAO+CSD group was significantly increased(P<0.05),compared with the MCAO+CSD group,the apoptosis index of neural cells in each phase of the electro-acupuncture group was significantly decreased(P<0.05).(3)Compared with the sham-operation group,the

关 键 词：电针 水沟穴 急性脑梗死 远隔功能障碍 皮层扩散性抑制 细胞凋亡 Na^(+)-K^(+)-ATP酶 单羧酸转运体1(MCT1) 胶质纤维酸性蛋白(GFAP) 大鼠 

分 类 号：R285.5[医药卫生—中药学]