柴胡加龙骨牡蛎汤对急性心肌梗死合并焦虑大鼠海马A型利钠肽及其受体的调节作用  

Regulating Effect of Chaihu Jia Longgu Muli Decoction on Hippocampal Atrial Natriuretic Peptide(ANP)and Its Receptor in Rats with Acute Myocardial Infarction Combined with Anxiety

作　　者：王威 陈雅丽 张秀静 王帅 马迪 赵海滨[2] WANG Wei;CHEN Ya-Li;ZHANG Xiu-Jing;WANG Shuai;MA Di;ZHAO Hai-Bin(Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;Beijing University of Traditional Chinese Medicine Oriental Hospital,Beijing 100078,China)

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]北京中医药大学东方医院,北京100078

出　　处：《广州中医药大学学报》2022年第11期2615-2621,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：北京市自然科学基金资助项目(编号:7202126);中央高校基本科研业务费专项资金资助项目(编号:2019-JYB-JS-181);中央高校基本科研业务费专项资金资助项目(编号:2019-JYB-XS-233)。

摘　　要：【目的】探讨柴胡加龙骨牡蛎汤对急性心肌梗死(AMI)合并焦虑大鼠的治疗作用及机制。【方法】将18只SD大鼠分为假手术组、复合模型组及中药组,每组6只。复合模型组和中药组采用不确定空瓶刺激法结合冠状动脉左前降支结扎法构建AMI合并焦虑模型。假手术组仅穿线后打松结,余步骤相同。造模结束后,中药组给予柴胡加龙骨牡蛎汤灌胃,复合模型组和假手术组给予生理盐水灌胃。给药结束后,进行超声心动检查及行为学实验,采用尼氏染色法观察大鼠海马病理变化,分别采用实时聚合酶链反应(RT-PCR)法、Western Blot法检测海马A型利钠肽(ANP)及利尿钠肽A型受体(NPRA)的mRNA、蛋白表达水平。【结果】(1)超声心动图结果显示,与复合模型组比较,中药组左室射血分数(EF)及左室短轴率(FS)值升高(P<0.01),左室收缩末内径(LVIDs)和左室舒张末内径(LVIDd)水平下降(P<0.01)。(2)旷场实验结果显示,与复合模型组比较,中药组大鼠水平运动得分和垂直运动得分有所上升(P<0.05);高架十字迷宫实验结果显示,与复合模型组比较,中药组的大鼠更倾向于待在开臂里,待在开臂的次数和时间比值均明显升高(P<0.05)。(3)海马尼氏染色结果显示,与复合模型组比较,中药组大鼠海马区尼氏小体明显增多。(4)海马ANP及NPRA水平:与复合模型组比较,中药组大鼠ANP与NPRA mRNA、蛋白相对表达量均明显上升(P<0.05)。【结论】柴胡加龙骨牡蛎汤可有效改善AMI合并焦虑大鼠心功能及焦虑状态,其机制与其增加海马ANP及其受体表达水平有关。Objective To investigate the therapeutic effect and mechanism of Chaihu Jia Longgu Muli Decoction in rats with acute myocardial infarction(AMI)combined with anxiety.Methods Eighteen SD rats were divided into the sham-operation group,the compound model group and the Chinese medicine group,with six rats in each group.The composite model group and the Chinese medicine group adopted uncertain empty bottle stimulation and the left anterior descending branch of coronary artery ligation to establish the AMI combined with anxiety model.The sham-operation group tied loose knot after threading,and the rest of the procedures were the same as in the composite model group and the Chinese medicine group.After the modeling,the Chinese medicine group was given gastric gavage with Chaihu Jia Longgu Muli Decoction,while the composite model group and the shamoperation group were given normal saline gavage.After drug administration,echocardiography and behavioural experiments were performed,and Nissl’s staining was used to observe the pathological changes in the hippocampus of the rats.The m RNA and protein expression levels of hippocampal atrial natriuretic peptide(ANP)and natriuretic peptide receptor A(NPRA)were detected by real-time polymerase chain reaction(RT-PCR)and Western Blot,respectively.Results(1)The echocardiographic results showed that the left ventricular ejection fraction(EF)and left ventricular fractional shortening(FS)values were increased(P<0.01)and the left ventricular end-systolic internal dimension(LVIDs)and left ventricular end-diastolic internal dimension(LVIDd)were decreased(P<0.01)in the Chinese medicine group compared with the composite model group.(2)The results of the open field test showed that the horizontal movement score and vertical movement score of rats in the Chinese medicine group were increased compared with that of the composite model group(P<0.05);the results of the elevated plus maze test showed that rats in the Chinese medicine group tended to stay in the open arm more compared with that o

关 键 词：柴胡加龙骨牡蛎汤 急性心肌梗死 焦虑 A型利钠肽(ANP) 利尿钠肽A型受体(NPRA) 大鼠 

分 类 号：R285.5[医药卫生—中药学]