小分子干扰RNA抑制鼻黏膜上皮细胞血管紧张素转换酶2的表达  

Small-molecule interference with RNA inhibits the expression of angiotensin-converting enzyme 2 in nasal mucosal epithelial cells

作　　者：鲍羿岐 刘洋[1] 崔娜[1] 朱学伟[1] BAO Yi-qi;LIU Yang;CUI Na(Department of Otorhinolaryngology Head and Neck Surgery,China-Japan Union Hospital of Jilin University,Changchun130033,China)

机构地区：[1]吉林大学中日联谊医院耳鼻咽喉头颈外科,吉林长春130033

出　　处：《中国实验诊断学》2022年第11期1702-1705,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅社发处项目资助(20210203077SF)。

摘　　要：目的通过小干扰RNA(siRNA)沉默鼻黏膜上皮细胞(HNECs)上的血管紧张素转化酶2(ACE2)受体,观察siRNA干扰鼻黏膜上皮细胞的ACE2表达后对其下游信号通路的影响。方法应用脂质体法将si-ACE2转染到HNECs细胞内,通过RT-PCR方法检测各组细胞中ACE2mRNA的表达。Western blot检测各组细胞ACE2蛋白表达水平以及下游信号通路ERK1/2,STAT3蛋白的磷酸化水平。结果RT-PCR结果显示si-ACE2组细胞中ACE2的mRNA水平明显低于空白对照组,结果具有统计学意义(P<0.05)。Western blot检测结果提示si-ACE2组细胞中p-ERK1/2、p-STAT3蛋白表达水平明显增加,结果具有统计学意义(P<0.05)。结论鼻黏膜上皮细胞表达ACE2受体,通过siRNA重组质粒可以下调ACE2的表达,但同时提高了鼻黏膜上皮细胞其下游信号通路ERK1/2及STAT3蛋白的磷酸化水平。Objective To observe the effect of small interfering RNA(siRNA)on expression of angiotensin converting enzyme 2(ACE2)and downstream signals in nasal mucosal epithelial cells(HNECs).Methods The si-ACE2was transfected into HNECs cells,and the expression of ACE2mRNA in each group was detected by RT-PCR.The ACE2protein expression level and the phosphorylation level of the downstream signaling pathway,ERK1/2,and STAT3protein were determined by Western blot.Results The RT-PCR results showed that the mRNA level of ACE2in the siACE2group of cells was significantly lower than that in the blank control group,and the results were statistically significant(P<0.05).The Western blot test results indicated that the p-ERK1/2and p-STAT3protein expression levels increased significantly in the si-ACE2group of cells,and the results were statistically significant(P<0.05).Conclusion Nasal mucosa epithelial cells express ACE2receptor,which can downregulate ACE2expression by siRNA recombinant plasmids,but also increase the phosphorylation level of ERK1/2and STAT3protein in nasal mucosa epithelial cells.

关 键 词：血管紧张素转换酶2 小干扰RNA 鼻黏膜上皮细胞 

分 类 号：R446[医药卫生—诊断学]