miR-1260b经MAPK/ERK途径促进再生障碍性贫血小鼠骨髓造血功能恢复  

Mir-1260b promotes the recovery of bone marrow hematopoietic function in aplastic anemia mice via MAPK/ERK pathway

作　　者：党青华 罗君 王敏 王岑 李功娟 钟舍兰 DANG Qing-hua;LUO Jun;WANG Min(Pediatric Internal Medicine of Xi'an International Medical Center Hospital,Xi'an 710119,China)

机构地区：[1]西安国际医学中心医院小儿内科,西安陕西710119

出　　处：《中国实验诊断学》2022年第12期1816-1822,共7页Chinese Journal of Laboratory Diagnosis

基　　金：陕西省卫生健康科研项目2021YB056。

摘　　要：目的分析微小RNA-1260b(Microrna-1260b,miR-1260b)是否经丝裂原激活的蛋白激酶/胞外信号调节的蛋白激酶(Mitogen activated protein kinase/extracellular signal regulated protein kinase,MAPK/ERK)途径促进再生障碍性贫血小鼠骨髓造血功能恢复。方法选取11周龄近交系雄性C57BL/6J小鼠6只,作为供体鼠,选取8~12周龄近交系雄性CB6F1小鼠60只,作为实验鼠。取所供体鼠制备泛T淋巴细胞,实验鼠移植泛T淋巴细胞并射线照射诱导建立再生障碍性贫血。将CB6F1小鼠分为4组,即为正常组、模型组、过表达组、沉默组,将10μl miR-1260b过表达、miR-1260b沉默载体的慢病毒悬液注射于过表达组、沉默组尾部,空白组、模型组不做任何处理,7 d后观察骨髓象、骨髓有核细胞、外周血象、造血相关因子、免疫功能及MAPK/ERK信号通路蛋白表达情况。结果在外周血象(WBC、PLT、Hb)、造血相关因子(EPO、TPO、VEGF)、骨髓有核细胞数、免疫功能(CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))、MAPK/ERK信号通路蛋白表达(p-MEK、p-ERK、p-JNK、p-P38)上对比,模型组、过表达组、沉默组WBC、PLT、Hb、VEGF水平、CD4^(+)、CD4^(+)/CD8^(+)、骨髓有核细胞数、p-MEK、p-ERK低于正常组(P<0.05)。过表达组WBC、PLT、Hb、VEGF水平、CD4^(+)、CD4^(+)/CD8^(+)、骨髓有核细胞数、p-MEK、p-ERK低于模型组(P<0.05)。沉默组WBC、PLT、Hb、VEGF水平、CD4^(+)、CD4^(+)/CD8^(+)、骨髓有核细胞数、p-MEK、p-ERK高于模型组(P<0.05)。沉默组WBC、PLT、Hb、VEGF水平、CD4^(+)、CD4^(+)/CD8^(+)、骨髓有核细胞数、p-MEK、p-ERK高于过表达组(P<0.05)。模型组、过表达组、沉默组EPO、TPO、CD8^(+)、p-JNK、p-P38高于正常组(P<0.05)。过表达组EPO、TPO、CD8^(+)、p-JNK、p-P38高于模型组(P<0.05)。沉默组EPO、TPO、CD8^(+)、p-JNK、p-P38低于模型组(P<0.05)。沉默组EPO、TPO、CD8^(+)、p-JNK、p-P38低于过表达组(P<0.05)。结论沉默miR-1260b可促进再生障碍性贫血Objective To analyze whether microrna-1260 b(mir-1260 b)promotes the recovery of bone marrow hematopoietic function in aplastic anemia mice through mitogen activated protein kinase/extracellular signal regulated protein kinase(MAPK/ERK)pathway.Methods Six 11-week-old inbred male C57 BL/6 J mice were selected as donor mice,and 608-to 12-week-old inbred male CB6 F1 mice were selected as experimental mice.Pan-T lymphocytes were prepared from donor mice,and experimental mice were transplanted with pan-T lymphocytes with radiation irradiation to establish aplastic anemia.CB6 F1 mice were divided into four groups,namely normal,model,overexpression and silent groups,and the lentiviral suspension with 10μl miR-1260 b overexpression and miR-1260 b silent vector was injected into the tail of overexpression group and silent group without any treatment.After 7 d,bone marrow,bone marrow nucleated cells,peripheral blood,hematopoietic-related factors,immune function and MAPK/ERK signaling protein expression were observed.Results In peripheral blood(WBC,PLT,Hb),hematopoietic-related factors(EPO,TPO,VEGF),number of bone marrow nucleated cells,immune function(CD4^(+),CD8^(+),CD4^(+)/CD8^(+)),MAPK/ERK signaling pathway protein expression(p-MEK,p-ERK,p-JNK,p-P38),model group,overexpression group,silence group WBC,PLT,Hb,VEGF levels,CD4^(+),CD4^(+)/CD8^(+),bone marrow nucleated cells,p-MEK,p-ERK was lower than the normal group(P<0.05).The levels of WBC,PLT,Hb,VEGF,CD4^(+),CD4^(+)/CD8^(+),bone marrow nucleated cells,p-MEK and p-ERK in the overexpression group were lower than those in the model group(P<0.05).The levels of WBC,PLT,Hb,VEGF,CD4^(+),CD4^(+)/CD8^(+),bone marrow nucleated cells,p-MEK and p-ERK in the silence group were higher than those in the model group(P<0.05).The levels of WBC,PLT,Hb,VEGF,CD4^(+),CD4^(+)/CD8^(+),bone marrow nucleated cells,p-MEK and p-ERK in the silence group were higher than those in the overexpression group(P<0.05).EPO,TPO,CD8^(+),p-JNK and p-P38 in model group,overexpression group and silence group w

关 键 词：微小RNA-1260b 经丝裂原激活的蛋白激酶/胞外信号调节的蛋白激酶 再生障碍性贫血 骨髓造血功能 

分 类 号：R551[医药卫生—血液循环系统疾病]