熔盐法制备含钴氯磷灰石及其抗氧化性能和细胞相容性研究  

Cobalt-incorporated Chlorapatite: Preparation by Molten Salt Method, Anti-oxidation and Cytocompatibility

作　　者：舒朝琴 朱敏 朱钰方[2] SHU Chaoqin;ZHU Min;ZHU Yufang(School of Materials and Chemistry,University of Shanghai for Science and Technology,Shanghai 200093,China;StateKey Laboratory of High Performance Ceramics and Superfine Microstructure,Shanghai Institute of Ceramics,ChineseAcademy of Sciences,Shanghai 200050,China)

机构地区：[1]上海理工大学材料与化学学院,上海200093 [2]中国科学院上海硅酸盐研究所,高性能陶瓷与超微结构国家重点实验室,上海200050

出　　处：《无机材料学报》2022年第11期1225-1235,共11页Journal of Inorganic Materials

基　　金：国家自然科学基金(51872185,52072246)。

摘　　要：生物活性陶瓷骨修复材料虽然具有优异的成骨性能,但缺乏抗氧化应激的能力,妨碍骨修复进程。本研究以β相磷酸三钙(β-TCP)粉体为原料,采用LiCl-KCl熔盐体系,以六水合氯化钴(CoCl_(2)·6H_(2)O)为钴源,利用熔盐法制备出含钴氯磷灰石(Co-MS-TCP)。通过Co-MS-TCP粉体清除过氧化氢(H_(2)O_(2))分析了含钴氯磷灰石的抗氧化能力;通过细胞活性、胞内活性氧(ROS)含量变化评价了材料的细胞相容性和细胞水平抗氧化性能。结果表明,熔盐处理β-TCP粉体能够制备含钴氯磷灰石,钴含量随CoCl_(2)·6H_(2)O加入量增加而增大;H_(2)O_(2)清除能力随氯磷灰石中钴含量的增加而增强, 6 h内对H_(2)O_(2)的清除率可达90%以上。细胞实验证实,含钴氯磷灰石具有良好的细胞相容性和抗氧化性能,1.5mg·mL^(-1)加3%Co盐的MS-TCP(3%Co-MS-TCP)即可保证软骨细胞和骨髓间充质干细胞存活率大于85%,并且3%Co-MS-TCP可有效清除H_(2)O_(2),使得细胞内ROS含量显著降低。因此,通过熔盐法制备含钴生物活性陶瓷是实现抗氧化应激的一种有效途径,这也为开发催化活性高、生物相容好的功能化生物活性陶瓷提供了新的策略。Orthopedic surgery and postoperative inflammation are easy to induce oxidative stress,which hinders the process of bone repair.Bioactive ceramics have excellent osteogenic properties,but lack the ability to resist oxidative stress.Therefore,it is of great significance to develop a bioactive ceramic material with antioxidant function.Here,cobalt-incorporated chloroapatite(Co-MS-TCP)was prepared by a molten salt method,in which the mixture of lithium chloride and potassium chloride was used as a molten salt system,andβ-phase tricalcium phosphate(TCP)and cobalt chloride hexahydrate(CoCl_(2)·6H_(2)O)were used as raw material and cobalt source,respectively.The antioxidant ability of Co-MS-TCP was determined by catalyzing H_(2)O_(2) clearance.The cytocompatibility and anti-oxidation of Co-MS-TCP were further evaluated by analyzing the changes of cell viability and intracellular reactive oxygen species(ROS).Results showed that Co-MS-TCP with controllable cobalt content can be prepared by a molten salt method with changing the addition amount of CoCl_(2)·6H_(2)O source.The scavenging capacity of H_(2)O_(2) increased with the increase of cobalt content in chlorapatite,and more than 90%of H_(2)O_(2) could be scavenged within 6 h due to the catalytic activity of Co-MS-TCP.Furthermore,cell experiments confirmed the cytocompatibility and antioxidative property of Co-MS-TCP.3%Co-MS-TCP at a concentration of 1.5 mg·mL^(-1) could still ensure the survival rate of bone marrow mesenchymal stem cells and chondrocytes to be higher than 85%,and 3%Co-MS-TCP can also significantly reduce the content of intracellular ROS for the H_(2)O_(2)-stimulated cells.Therefore,molten salt method is an effective way to prepare cobalt-incorporated bioactive ceramics with antioxidative property,which also provides a promising strategy for the development of functional bioactive ceramics with high catalytic activity and biocompatibility.

关 键 词：生物活性陶瓷 熔盐法 钴 抗氧化 

分 类 号：TQ174[化学工程—陶瓷工业]