菠萝蛋白酶铜簇荧光猝灭法检测叶酸  

Bromelain-templated copper cluster probe for fluorescence turn-off determination of folic acid

作　　者：易雨欣 吴思思 牟娇 符运雪 刘璐航 钱多 梁好 杨胜园 YI Yu-xin;WU Si-si;MOU Jiao;FU Yun-xue;LIU Lu-hang;QIAN Duo;LIANG Hao;YANG Sheng-yuan(Department of public health laboratory sciences,school of public health,Hengyang Medical School,University of South China,Hengyang 421001,China)

机构地区：[1]南华大学衡阳医学院,公共卫生学院卫生检验与检疫系,湖南衡阳421001

出　　处：《化学研究与应用》2023年第1期59-65,共7页Chemical Research and Application

基　　金：国家自然科学基金项目(82073604,81903369)资助。

摘　　要：本文以菠萝蛋白酶为模板,水合联氨为还原剂,采用水热法合成了一种能发射橙色荧光的菠萝蛋白酶铜纳米簇(Bro-CuNCs),该Bro-CuNCs的最大激发和发射波长分别为325 nm和585 nm,基于Bro-CuNCs和叶酸之间的内滤效应(IFE),叶酸可导致Bro-CuNCs的荧光发生猝灭。在最佳实验条件下,叶酸在5.0×10^(-8)~2.0×10^(-6) mol·L^(-1)的浓度范围内与荧光猝灭度ΔF/F_(0)呈线性关系,回归方程为ΔF/F 0=1.56c(0.1μM)+0.046,相关系数r=0.9980,检出限为1.85×10^(-8) mol·L^(-1),加标回收率介于98.3%~103.6%之间,相对标准偏差(RSD)为1.2%~4.0%,说明该方法准确度好,精密度高。Bromelain copper nanoclusters(Bro-CuNCs)with emitting orange fluorescence were synthesized by a hydrothermal method using bromelain as a template and hydrazine hydrate as a reducing agent in this work.The Bro-CuNCs have a strong symmetric emission at 585 nm with a maximum excitation at 325 nm.Folic acid can lead to the fluorescence quenching of Bro-CuNCs,based on the inner filter effect(IFE)between Bro-CuNCs and folic acid.Under the optimal experimental conditions,folic acid showed a linear relationship with the fluorescence quenching degreeΔF/F_(0 )in the concentration range of 5.0×10^(-8)~2.0×10^(-6) mol·L^(-1).The regression equation wasΔF/F 0=1.56c(0.1μM)+0.046,r=0.9980.The detection limit was 1.85×10^(-8) mol·L^(-1),the recovery was between 98.3%and 103.6%.The relative standard deviation(RSD)was 1.2%~4.0%,indicating that the method had good accuracy and high precision.

关 键 词：铜纳米簇 叶酸 荧光猝灭 

分 类 号：O657.3[理学—分析化学]