基于mtDNA D-Loop区和Cytb基因的清水江斑鳜群体遗传多样性分析  

Analysis of genetic diversity of Siniperca scherzeri population in Qingshui River based on mitochondrial Cytb and D-Loop sequences

作　　者：麻智芳 潘秋芝 安苗[1] 李珊 黄胜[1] 余科 MA Zhifang;PAN Qiuzhi;AN Miao;LI Shan;HUANG Sheng;YU Ke(College of Animad Sience,Guishou Uniesity,Guiyang 550025,China;Guizhou Vocationad College of Agriculure,Guiyang 551499,China)

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]贵州农业职业学院,贵阳551499

出　　处：《海洋渔业》2022年第6期657-669,共13页Marine Fisheries

基　　金：国家自然科学基金(31660741);贵州省科技计划项目([2017]5788)。

摘　　要：为了解贵州省境内清水江流域野生斑鳜(Siniperca scherzeri)的遗传多样性现状,利用mtDNA Cytb基因和D-Loop区测序技术,分析了清水江287尾野生斑鳜的遗传结构及其遗传多样性。结果表明:分别获得287条斑鳜的完整Cytb基因序列(1141 bp)和部分D-Loop区序列(840~841 bp);两个基因序列的碱基组成中A+T含量分别为51.6%和64.1%,均高于C+G含量的48.4%和35.9%,多态位点分别有23和44个,单倍型数分别为25和43种;单倍型多样性指数(核苷酸多样性指数)分别为0.821±0.018(0.0020±0.0001)和0.919±0.009(0.0083±0.0003);群体内平均遗传距离为0.0021和0.0087;AMOVA分析显示,群体内个体间变异是变异的主要来源(Cytb基因为97.54%,D-Loop区为97.32%);NJ系统发生树显示,Cytb和D-Loop区基因都聚为两支,两支的单倍型在河流的上、中、下游均有分布;清水江野生斑鳜群体的mtDNA Cytb的Fu’s Fs值呈显著负值(-0.9553,P=0.007),检测出了种群扩张,据公式推算,清水江斑鳜种群于0.176Ma年前发生了种群扩张,而D-Loop区符合中性进化的假设,未检测到种群扩张。综上所述,清水江野生斑鳜没有发生明显的遗传分化现象,遗传多样性高,呈现出较高的单倍型和核苷酸多态性。研究可为清水江野生斑鳜资源保护和持续发展与利用提供基础参考材料。Qingshui River is located in the northern part of the eastern section of Miaoling Mountain range,which is the slope of the transition from the Yunnan-Guizhou Plateau to the hills of Hunan and Guangxi,and it is the upper reaches of Yuanjiang River in Dongting Lake Water System of the Yangtze River basin,and it is also the second largest river in Guizhou Province.In order to analyze the genetic diversity and population genetic structure of wild Siniperca scherzeri population in Qingshui River basin,and to provide a theoretical basis for conservation management of the wild resources,we have set up a total of ten sampling sites in the upper,middle and lower reaches of Qingshui River and collected 287 individuals of wild Siniperca scherzeri.Genetic diversity and phylogenetic relationshihs of 287 wild Siniperca scherzeri were investigated based on mitochondrial cytochrome b(Cytb)and control region(D-Loop)sequences.The completed mt DNA Cytb gene sequence(1141 bp)and partial D-Loop control region sequence(840-841 bp)of wild Siniperca scherzeri were PCR amplified,sequenced,and analyzed.The preliminary findings were as follows:1)The average contents of A,T,G and C in Cytb sequences were 24.3%,27.3%,15.5%and 32.4%,respectively,and the corresponding contents off our bases in D-Loop sequences were 34.0%,30.1%,15.8%and 20.1%,respectively.Meanwhile,the average content of A+T was 51.6%(Cytb)and 64.1%(D-Loop)respectively,both higher than the average C+G content of 48.4%(Cytb)and 35.9%(D-Loop),and the proportion of base G was the lowest,respectively,the data showed a bias against G,and the proportion of A to T in Cytb and D-Loop gene sequence was a little different.In addition,23 variation sites were detected in Cytb gene sequences,of which 16 were parsimony informative sites and 7 were singleton variable sites,no base insertions or deletions,and a base conversion/reversal ratio of 4.60.While 44 variation sites were detected in D-Loop gene sequences,of which 39 were parsimony informative sites and 5 were singleton variable sites,n

关 键 词：斑鳜 遗传多样性 CYTB D-LOOP 

分 类 号：Q349[生物学—遗传学]