基于耳石微化学特征甄别黑鲷放流群体和野生群体的初步研究  

Distinguishing wild from released juvenile Sparus macrocephalus with otolith chemical signatures

作　　者：张翼[1] 张辉[1] 姜亚洲[1] 凌建忠[1] 刘尊雷[1] 李圣法[1] ZHANG Yi;ZHANG Hui;JIANG Yazhou;LING Jianzhong;LIU Zunlei;LI Shengfa(Key Labortory of East China Sea Fishery Resources Expliation and Uriliation,Ministry of Agriculure and Rrural Affairs,East China Sea Fiheries Research Instiule,Chinese Academy of Fiheris Scienes,Shanghai 200090,China)

机构地区：[1]中国水产科学研究院东海水产研究所,农业农村部东海渔业资源开发利用重点实验室,上海200090

出　　处：《海洋渔业》2022年第6期680-688,共9页Marine Fisheries

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项(2020YFD0900804);中央级公益性科研院所基本业务费专项基金项目(2015T09);中国水产科学研究院基本科研业务费(2016HY-ZD0102)。

摘　　要：基于耳石微化学特征研究甄别黑鲷(Sparus macrocephalus)放流群体和野生群体的可行性,并根据该特征对象山港捕获的黑鲷成鱼进行了群体判别。研究结果显示,向天然海水中加入适当淡水后形成养殖海水,养殖海水的Ba离子浓度显著高于天然海水(P<0.05);养殖黑鲷的耳石核心位置的Ba/Ca比值(C)、全耳石Ba/Ca比值(X)及Ba元素变异系数(CVI)均显著高于野生黑鲷(P<0.05)。耳石上的Ba/Ca比值可作为识别养殖黑鲷和野生黑鲷的微化学标志。以此为标志对象山港捕获的20尾黑鲷成鱼进行群体判别,结果表明2尾黑鲷为放流群体。A central goal of stock enhancement is to increase species abundance and potential harvest by supplementing recruitment of wild populations.The black sea bream Sparus macrocephalus represents very important recreational fishery along China’s coastline and is stocked in coastal bays to enhance natural fish abundance.To distinguish aquacultured fish from wild population is essential for evaluating the potential stock enhancement.Otolith chemistry signatures are regarded as natural tags to distinguish wild from released Sparus macrocephalus.From May to June 2019,before the enhancement and releasing of Sparus macrocephalus in Xiangshan Bay,juvenile samples were collected with gill nets,and water temperature and salinity of sampling sites were recorded.The body length of collected Sparus macrocephalus was 3.05-3.89 cm with a sample size of 20.The samples captured were identified to be wild population according to sampling time and body length.During the rearing of Sparus macrocephalus,fresh water was pumped into the seawater every day to adjust the salinity of cultured seawater,and the salinity of cultured seawater was kept at a suitable range of 18-25 for at least 30 days.Thirty cultured fish were collected.In August 2020,adult Sparus macrocephalus samples were collected from Xiangshan Bay,with a body length of 10.6-19.5 cm,and the sample size was 20.Samples of natural seawater,fresh water and cultured seawater were collected randomly for 5 days,filtered by microporous filter membrane(0.45μm)to remove impurities,collected and stored in plastic bottles,and brought back to the laboratory for detection of Sr and Ba concentrations.From each individual,sagittal otolith pairs were extracted from the fish,cleaned with H_(2)O_(2),rinsed with 18.2 MΩMQ water and dried.Sagittae were embedded in araldite resin,ground on a sagittal plane to expose their primordium and polished with diamond papers of decreasing grain diameter.Finally,the otoliths were double rinsed with 18.2 MΩMQ water,dried prior to LA-ICPMS analysis.A cont

关 键 词：黑鲷 耳石 钡 微化学标志 元素分析 

分 类 号：S931[农业科学—渔业资源]