热应激对猪颗粒细胞蛋白质表达谱的影响  

Effects of heat stress on protein expression profiles in porcine granulosa cells

作　　者：王泽平 沈婕 赵为民[2] 付言峰[2] 李碧侠[2] 任守文[2] 程金花[2] 李辉[2] WANG Ze-ping;SHEN Jie;ZHAO Wei-min;FU Yan-feng;LI Bi-xia;REN Shou-wen;CHENG Jin-hua;LI Hui(Suqian Institute of Agricultural Sciences,Jiangsu Academy of Agricultural Sciences,Suqian 223800,China;Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Animal Science and Technology,Guangxi University,Nanning 530005,China)

机构地区：[1]江苏省农业科学院宿迁市农业科学研究所,江苏宿迁223800 [2]江苏省农业科学院畜牧研究所,江苏南京210014 [3]广西大学动物科学技术学院,广西南宁530005

出　　处：《江苏农业学报》2022年第6期1569-1577,共9页Jiangsu Journal of Agricultural Sciences

基　　金：宿迁市自然科学资金项目(K202108);江苏省自然科学基金项目(BK20211140)。

摘　　要：为系统探究热应激对猪颗粒细胞蛋白质表达的影响,本研究将分离培养的猪颗粒细胞分别在37℃(对照组)和41℃(热应激组)处理后,用同位素标记相对和绝对定量(iTRAQ)技术对细胞的蛋白质差异表达情况进行分析,并对差异表达蛋白质进行GO功能注释和KEGG通路分析。本研究共鉴定到5 451个蛋白质,差异表达蛋白质289个,其中上调表达蛋白质162个,下调表达蛋白质127个。对筛选出的差异表达蛋白质进行KEGG通路分析发现,差异表达蛋白质主要富集到蛋白质加工、胞吞、RNA降解、氧化磷酸化、嘌呤代谢、泛素化蛋白质降解、次级代谢产物的生物合成、糖酵解/糖异生以及胞间紧密连接等信号通路中。说明这些差异表达蛋白质及其富集的通路可能参与热应激对猪颗粒细胞功能影响的过程中。因此,本研究结果为深入了解热应激导致母猪繁殖障碍的分子机理提供了参考。To systematically investigate the influence of heat stress on protein expression of porcine granulosa cells, isolated porcine granulosa cells were treated at 37 ℃(control group) and 41 ℃(heat stress group) in vitro. Subsequently, isobaric tags for relative and absolute quantification(iTRAQ) technique was used to analyze differentially expressed proteins in cells, and all the differentially expressed proteins were further analyzed by GO functional annotation and KEGG pathway analysis. The results showed that, a total of 5 451 proteins were identified, in which 289 were differentially expressed proteins. Among the differentially expressed proteins, 162 were up-regulated and 127 were down-regulated. Results of KEGG pathway analysis on the screened differentially expressed proteins showed that, they mainly gathered into signal pathways such as protein processing, endocytosis, RNA degradation, oxidative phosphorylation, purine metabolism, ubiquitin mediated proteolysis, biosynthesis of secondary metabolites, glycolysis/gluconeogenesis and intercellular tight junction. The results indicated that, the differentially expressed proteins and their enriched pathways may be involved in the process in which porcine granulosa cells functions were influenced by heat stress. Therefore, the results of this study can provide reference for in-depth understanding of the molecular mechanisms for heat stress-induced porcine reproductive failure.

关 键 词：同位素标记相对和绝对定量(iTRAQ) 猪 热应激 颗粒细胞 

分 类 号：S828[农业科学—畜牧学]