醋栗番茄LA2093渐渗系群体苗期耐盐性评价  

Evaluation of salt tolerance of introgression line population of Solanum pimpinellifolium LA2093 at seedling stage

作　　者：李浩龙 周蓉[1] 蒋芳玲[1] 文军琴 刘帅[1] 吴震[1] LI Hao-long;ZHOU Rong;JJANG Fang-ling;WEN Jun-qin;LIU Shuai;WU Zhen(College of Horticulture,Nanjing Agricultural University/Key Laboratory of Biology and Germplasm Innovation of Horticultural Crops in East China,Ministry of Agriculture and Rural Affairs,Nanjing 210095,China)

机构地区：[1]南京农业大学园艺学院/农业农村部华东地区园艺作物生物学与种质创新重点实验室,江苏南京210095

出　　处：《江苏农业学报》2022年第6期1620-1626,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金联合基金项目(U1903106);中央高校基本科研业务费项目(KYZZ2021004);江苏高校优势学科建设工程项目(PAPD)。

摘　　要：以栽培番茄(Solanum lycopersicum) Jina为母本,醋栗番茄(Solanum pimpinellifolium) LA2093为父本,构建渐渗系(introgression line,IL) BC3F3代群体。以该群体的19个渐渗系株系及亲本为试验材料,调查盐胁迫(200mmol/L Na Cl)处理下番茄苗期基于7个指标(株高、茎粗、整株鲜质量、整株干质量、叶绿素相对含量、丙二醛含量和过氧化氢含量)的耐盐性系数。通过对各指标对应的耐盐性系数进行相关性分析、主成分分析和隶属函数分析,综合评价各番茄材料的耐盐性。对各番茄材料的耐盐性综合评价值(D)进行聚类分析,筛选出3个耐盐株系(IL-6、IL-9和IL-12);通过逐步回归分析建立番茄苗期耐盐性预测方程:D=1.155α2+0.576α3+0.525α6-1+0.341α7-1-1.582,并筛选出茎粗、整株鲜质量、丙二醛含量和过氧化氢含量作为番茄苗期耐盐性评价指标。通过测定番茄幼苗的茎粗、整株鲜质量、丙二醛含量和过氧化氢含量,进而分析相应的耐盐系数,可以对番茄耐盐性进行预测。BC3F3population of introgression line (IL) was constructed with cultivar tomato Jina (Solanum lycopersicum) as female parent and wild tomato LA2093 (Solanum pimpinellifolium) as male parent.By using 19 individual plants from BC3F3ILs and their parents as experimental materials,salt tolerance coefficients derived from seven indexes (plant height,stem diameter,plant fresh weight,plant dry weight,relative chlorophyll content,malondialdehyde content and hydrogen peroxide content) of tomato seedling under salt stress (200 mmol/L Na Cl) were investigated.The salt tolerance coefficients of each index were used to do correlation analysis,principal component analysis and affiliation function analysis to comprehensively evaluate the salt tolerance of each tomato material.Cluster analysis was performed on the comprehensive salt tolerance evaluation value (D) of each material,and three salt-tolerant strains (IL-6,IL-9 and IL-12) were selected.The salt tolerance prediction equation was established by stepwise regression analysis:D=1.155α2+0.576α3+0.525α6-1+0.341α7-1-1.582,and stem diameter,plant fresh weight,malondialdehyde content and hydrogen peroxide content were selected as the evaluation indexes of salt tolerance for tomato seedlings.Salt tolerance of tomato seedling can be predicted by measuring stem diameter,plant fresh weight,malondialdehyde content and hydrogen peroxide content and analyzing their related salt tolerance coefficients.

关 键 词：番茄 苗期 耐盐性 渐渗系 综合评价 

分 类 号：S641.2[农业科学—蔬菜学]