基于BSA-seq技术挖掘糙皮侧耳抗螨候选基因  

Candidate anti-mite gene detection by BSA-seq in Pleurotus ostreatus

作　　者：李辉平[1] 骆昕 侯子强 蒋宁[1] 林金盛[1] 侯立娟[1] 徐平 马林[1] 曲绍轩[1,2] LI Hui-ping;LUO Xin;HOU Zi-qiang;JIANG Ning;LIN Jin-sheng;HOU Li-juan;XU Ping;MA Lin;QU Shao-xuan(Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;School of Life Sciences,Jiangsu University,Zhenjiang 212013,China)

机构地区：[1]江苏省农业科学院蔬菜研究所,江苏省高效园艺作物遗传改良重点实验室,江苏南京210014 [2]江苏大学生命科学学院,江苏镇江212013

出　　处：《江苏农业学报》2022年第6期1648-1656,共9页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学青年基金项目(32001911);财政部和农业农村部国家现代农业产业技术体系资助项目(CARS-20)。

摘　　要：根据前期不同糙皮侧耳菌株抗螨性差异调查结果,构建了糙皮侧耳孢子单核体群体,群体内479个菌株抗螨性呈正态分布。利用BSA-seq技术对高抗和高感混池进行SNP和InDel位点的欧氏距离关联分析,将抗螨性候选基因定位到一条染色体上,2个相邻候选区域总长度为1.75 Mb,内有基因605个,其中非同义突变基因353个,移码突变基因89个。经功能注释以及GO和KEGG通路富集等分析,发现26个候选基因参与了信号传导、防御过程和次级代谢相关通路,推测这些候选基因可能与糙皮侧耳抗螨性相关。In our previous study, it was found that there were significant differences in the resistance to Tyrophagus putrescentiae among different strains of Pleurotus ostreatus. In this study, firstly, a spore monokaryotic population of P. ostreatus was rapidly constructed, and the 479 strains within the population were normally distributed for mite resistance. Secondly, the Euclidean distance association analysis of SNP and InDel sites was performed between the highly resistant and highly sensitive pools using BSA-seq technology. The candidate genes for mite resistance were located on one chromosome. The two adjacent candidate regions had a total length of 1.75 Mb and contained 605 genes, including 353 non-synonymous and 89 frameshift mutated genes. Finally, after functional annotation, GO and KEGG pathway enrichment analysis, 26 candidate genes were found to be involved in signal transduction, defense processes and secondary metabolism-related pathways. It was thus inferred that these candidate genes might be related to mite resistance.

关 键 词：糙皮侧耳(平菇) 抗螨候选基因 群体分离分析法 

分 类 号：S646.141[农业科学—蔬菜学]