地佐辛通过上调miR-204-3p减轻LPS诱导的心肌细胞H9C2损伤  

Dezocine reduces injury of LPS-induced cardiomyocyte H9C2 by up-regulating miR-204-3p

作　　者：鲁美静[1] 文怀昌[1] 汪懿[1] 王梦丽 张恺辰 陈永权[1] LU Meijing;WEN Huaichang;WANG Yi;WANG Mengli;ZHANG Kaichen;CHEN Yongquan(Department of Anesthesiology,the First Affiliated Hospital of Wannan Medical College,Wuhu 241000,China)

机构地区：[1]安徽省芜湖市皖南医学院弋矶山医院麻醉科,芜湖241000

出　　处：《中国免疫学杂志》2022年第20期2467-2472,共6页Chinese Journal of Immunology

摘　　要：目的:探讨地佐辛对脂多糖(LPS)诱导的心肌细胞损伤的影响及可能机制。方法:体外培养心肌细胞H9C2,分为Con组、LPS组、不同剂量(4、8、16μmol/L)地佐辛组、16μmol/L地佐辛+anti-miR-NC组和16μmol/L地佐辛+anti-miR-204-3p组,CCK-8检测细胞增殖,流式细胞仪检测细胞凋亡,Western blot检测CyclinD1、p21、Bcl-2和Bax蛋白表达,试剂盒检测细胞培养上清中LDH水平及细胞中MDA、SOD和GSH-Px水平,RT-qPCR检测细胞miR-204-3p表达。结果:与Con组比较,LPS组H9C2细胞OD值、CyclinD1和Bcl-2蛋白表达、SOD和GSH-Px活性及miR-204-3p表达降低(P<0.05),细胞凋亡率、p21和Bax蛋白表达及MDA和LDH含量升高(P<0.05)。与LPS组比较,不同剂量(4、8、16μmol/L)地佐辛组H9C2细胞OD值、CyclinD1和Bcl-2蛋白表达、SOD和GSH-Px活性及miR-204-3p表达升高(P<0.05),细胞凋亡率、p21和Bax蛋白表达及MDA和LDH含量降低(P<0.05),且呈剂量依赖性。与16μmol/L地佐辛+anti-miR-NC组比较,16μmol/L地佐辛+anti-miR-204-3p组H9C2细胞OD值、CyclinD1和Bcl-2蛋白表达、SOD和GSH-Px活性及miR-204-3p表达降低(P<0.05),细胞凋亡率、p21和Bax蛋白表达及MDA和LDH含量升高(P<0.05)。结论:地佐辛可能通过上调miR-204-3p表达促进LPS诱导的心肌细胞H9C2增殖,并抑制H9C2细胞凋亡和氧化应激,减轻LPS诱导的H9C2细胞损伤。Objective:To investigate effect of dezocine on injury of lipopolysaccharide(LPS)-induced cardiomyocyte and its possible mechanism.Methods:Cardiomyocytes H9C2 were cultured in vitro and divided into Con group,LPS group,different doses(4,8,16 μmol/L)of dezocine groups,16 μmol/L dezocine+anti-miR-NC group and 16 μmol/L dazocine+anti-miR-204-3p group.CCK-8 was used to detect cell proliferation;flow cytometry was used to detect cell apoptosis;Western blot was used to detect protein expressions of CyclinD1,p21,Bcl-2 and Bax;kits were used to detect level of LDH in cell culture supernatant and levels of MDA,SOD and GSH-Px in cells;RT-qPCR was used to detect expression of miR-204-3p in cells.Results:Compared with Con group,OD value,protein expressions of CyclinD1 and Bcl-2,activities of SOD and GSH-Px and expression of miR-204-3p of H9C2 cells in LPS group were decreased(P<0.05),while apoptosis rate,protein expressions of p21 and Bax,and contents of MDA and LDH were increased(P<0.05). Compared with LPS group,OD value,protein expressions of CyclinD1 and Bcl-2,activities of SOD and GSH-Px,and expression of miR-204-3p of H9C2 cells in different doses of dezocine(4,8,16 μmol/L)groups were increased(P<0.05),while apoptosis rate,protein expressions of p21 and Bax,and contents of MDA and LDH were decreased(P<0.05),which in a dose-dependent manner. Compared with 16 μmol/L dezocine+anti-miR-NC group,OD value,protein expressions of CyclinD1 and Bcl-2,activities of SOD and GSH-Px,and expression of miR-204-3p of H9C2 cells in 16 μmol/L dezocine+anti-miR-204-3p group were decreased(P<0.05),while apoptosis rate,protein expressions of p21 and Bax,and contents of MDA and LDH were increased(P<0.05).Conclusion:Dezocine may promote proliferation of LPS-induced cardiomyocytes H9C2 and inhibit H9C2 cell apoptosis and oxidative stress by up-regulating expression of miR-204-3p,which reduced injury of H9C2 cells induced by LPS.

关 键 词：地佐辛 miR-204-3p 心肌细胞 凋亡 氧化应激 

分 类 号：R542.2[医药卫生—心血管疾病]