甘肃地区转录因子Foxp3基因多态性与原因不明复发性流产的相关性研究  

Association between transcription factor Foxp3 gene polymorphism and susceptibility to unexplained recurrent spontaneous abortion in Gansu population

作　　者：刘倩 李亚梅[1] 王文第[1] 王燕侠[1] 毛宝宏[1] LIU Qian;LI Yamei;WANG Wendi;WANG Yanxia;MAO Baohong(Scientific Research Center,Gansu Provincial Maternity and Child Care Hospital,Lanzhou 730050,China)

机构地区：[1]甘肃省妇幼保健院科研中心,兰州730050

出　　处：《中国免疫学杂志》2022年第19期2384-2388,共5页Chinese Journal of Immunology

基　　金：甘肃省卫生行业科研计划项目(GSWSKY2018-54)资助。

摘　　要：目的:探讨转录因子Foxp3基因单核苷酸多态性和原因不明复发性流产(URSA)的相关性。方法:采用病例-对照研究方法选取甘肃地区2019年1月至2020年8月于甘肃省妇幼保健院就诊的URSA患者(URSA组)150例和同期正常妇女(对照组)150例作为研究对象,采用SNaPshot SNP分型技术对Foxp3基因的3个单核苷酸多态性位点(rs2232365、rs2280883和rs3761548)进行分型,比较分析其等位基因、基因型及所构建的单倍型在两组人群中的分布差异。结果:Foxp3基因rs2232365位点TT、TC、CC基因型在URSA组中的分布频率分别为38.0%、48.0%和14.0%,在对照组中的分布频率分别为35.3%、49.3%和15.3%。rs2280883位点TT、TC、CC基因型在URSA组中的分布频率分别为61.3%、34.0%和4.7%,在对照组中的分布频率分别为60.7%、34.0%和5.3%。rs3761548位点TT、GT、GG基因型在URSA组中的分布频率分别为4.0%、34.7%和61.3%,在对照组中的分布频率分别为5.3%、33.3%和61.3%。两组Foxp3基因3个位点基因型、等位基因分布差异均无统计学意义(P>0.05)。结论:转录因子Foxp3基因单核苷酸多态性可能与甘肃地区人群URSA发病不相关,有必要扩大样本量针对不同种族开展进一步研究。Objective:To investigate association between single nucleotide polymorphism of transcription factor Foxp3 gene and unexplained recurrent spontaneous abortion(URSA).Methods:A case-control study was conducted to select 150 cases of URSA patients(URSA group)and 150 cases of normal women(control group)in Gansu Provincial Maternity and Child Care Hospital from January 2019 to August 2020 as subjects.Three single nucleotide polymorphisms(rs2232365,rs2280883 and rs3761548)of Foxp3gene were genotyped by SNaPshot SNP typing technique.Distribution differences of alleles,genotypes and haplotypes in two groups were compared and analyzed.Results:Genotype distribution frequency of Foxp3 gene rs2232365 in URSA group were TT 38.0%,TC48.0%and CC 14.0%,those in control group were 35.3%,49.3%and 15.3%.Genotype distribution frequency of rs2280883 in URSA group were TT 61.3%,TC 34.0%and CC 4.7%,respectively,those in control group were 60.7%,34.0%and 5.3%.Genotype distribution frequency of rs3761548 in URSA group were TT 4.0%,GT 34.7%and GG 61.3%,while those in control group were 5.3%,33.3%and 61.3%.There was no significant difference in genotype and allele distribution of Foxp3 gene between two groups(P>0.05).Conclusion:Single nucleotide polymorphism of transcription factor Foxp3 gene may not be associated with pathogenesis of URSA in Gansu population,there is a need to expand sample size to conduct studies on different races for further validation.

关 键 词：转录因子 FOXP3 单核苷酸多态性 原因不明复发性流产 

分 类 号：R392.1[医药卫生—免疫学]