陕西省无偿献血人群中隐匿性丙型肝炎病毒感染情况研究  

Occult hepatitis C virus infection in volunteer blood donors in Shaanxi province

作　　者：郭燕[1] 贺晨[1] 赵晓华[1] 段勇[1] 徐华[1] 王文[1] 张国权[1] 李锦[1] GUO Yan;HEChen;ZHAO Xiaohua;DUAN Yong;XU Hua;WANG Wen;ZHANG Guoquan;LI Jin(Shaanxi Blood Center,Xi'an 710061,China)

机构地区：[1]陕西省血液中心,陕西西安710061

出　　处：《中国输血杂志》2022年第12期1256-1259,共4页Chinese Journal of Blood Transfusion

基　　金：陕西省自然科学基础研究计划(2015JM8393)。

摘　　要：目的 了解陕西省献血人群隐匿性丙型肝炎病毒感染(OCI)情况及病毒生物学特性。方法 收集2019年7~9月合格献血者血样112份,ALT单项不合格血样117份(ALT>50 U/L),HBsAg阳性血样46份,抗-HCV阳性血样75份,分离所有血样外周血淋巴细胞(PBMCs),提取总RNA,逆转录巢式PCR法(RT-Nested-PCR)扩增PBMCs中HCV RNA 5’非编码区,检测献血人群隐匿性丙型肝炎病毒感染情况;RT-Nested-PCR法扩增HCV RNA阳性标本core/E2区,确定HCV基因型和亚型。结果 抗-HCV阳性血样检出OCI 2份(2.67%,2/75), ALT不合格标本检出OCI 1份(0.85%,1/117),基因型均为HCV 1b;合格献血者和HBsAg阳性献血者均未检出OCI。结论 陕西省献血人群存在OCI,ALT筛查对献血人群OCI检出可能具有一定辅助作用。Objective To investigate the prevalence of occult hepatitis C virus infection(OCI) in Shaanxi blood donors and characterize the occult hepatitis C virus in this cohort. Methods Between July and September of 2019, 112, 117, 46, and 75 blood samples were obtained from eligible donors, alanine aminotransferase(ALT) elevated donors(ALT>50 U/L), hepatitis B virus surface antigen(HBsAg) positive donors, and anti-hepatitis C virus(anti-HCV) positive donors, respectively. Peripheral blood lymphocytes(PBMCs) were isolated from all the samples. HCV 5′UTR was amplified from total RNA of PBMCs using reverse transcription nested PCR(RT-Nested PCR) to detect the infection status of OCI in blood donors. HCV RNA core/E2 region was amplified from HCV RNA positive samples with RT-Nested PCR to determine HCV genotypes and subtypes via sequencing. Results Two(2.67%, 2/75) cases of OCI were identified in anti-HCV positive samples, 1(0.85%, 1/117) case of OCI was identified in abnormal ALT samples, and all OCI were genotyped as 1 b. OCI was not found in HBsAg positive and eligible blood donors. Conclusions Our findings suggest that OCI does exist among Shaanxi blood donors and that ALT may be useful in diagnosing OCI.

关 键 词：隐匿性丙肝 献血人群 外周血淋巴细胞 

分 类 号：R457.1[医药卫生—治疗学] R446[医药卫生—临床医学] R373.2