产黄青霉Penicillium chrysogenum S-3-25人工海水培养基发酵次级代谢产物研究  

The secondary metabolites isolated from Penicillium chrysogenum S-3-25 fermented in artificial seawater medium

作　　者：岳贤琳 杨郁 赵雪 陈波[3] 朱天骄[4] 张国刚[1] 李长伟 崔承彬 YUE Xian-lin;YANG Yu;ZHAO Xue;CHEN Bo;ZHU Tian-jiao;ZHANG Guo-gang;LI Chang-wei;CUI Cheng-bin(School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;State Key Laboratory of Toxicology and Medical Countermeasures,Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China;Key Laboratory for Polar Science of State Oceanic Administration,Polar Research Institute of China,Shanghai 200136,China;Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China)

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]军事医学研究院毒物药物研究所抗毒药物与毒理学国家重点实验室,北京100850 [3]中国极地研究中心国家海洋局极地科学重点实验室,上海200136 [4]中国海洋大学海洋药物教育部重点实验室医药学院,山东青岛266003

出　　处：《中国海洋药物》2022年第6期1-9,共9页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(81573300)资助。

摘　　要：目的阐明南极深海来源真菌产黄青霉Penicillium chrysogenum S-3-25人工海水培养基的发酵次级代谢产物及其活性。方法利用各种色谱技术分离纯化次级代谢产物,根据理化和波谱数据(核磁共振、质谱技术和Marfey分析)鉴定化合物结构,采用3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)法评价细胞毒活性。结果从真菌S-3-25人工海水培养基发酵产物中分离鉴定了8个单体化合物:L-Ala-2-aminobenzoic acid(1)、L-Ala-2-aminobenzoic acid-methyl ester(2)、2S-(2-hydroxypropanamido)benzamide(3)、3-(p-hydroxyphenyl)-N-methylpropionamide(4)、4-hydroxycinnamamide(5)、cyclo-(L-Pro-L-Tyr)(6)、脑苷脂A(7)及脑苷脂B(8)。细胞毒活性测试结果表明化合物1~8在50μmol/L浓度下对3种受试细胞(人乳腺癌MCF-7,人肺癌A549以及小鼠小胶质BV2细胞)的抑制率均低于50%。结论从极地深海来源真菌产黄青霉S-3-25人工海水培养基发酵产物中分离得到8个单体化合物,其中化合物1和2为新天然产物,未见有文献数据的报道。化合物1~8均未呈现较强的细胞毒活性。Objective To investigate the secondary metabolites of the fungus Penicillium chrysogenum S-3-25isolated from polar deep sea and fermented in artificial seawater medium.Methods The secondary metabolites were isolated by multiple separation techniques,the structures of the isolated compounds were identified according to the physicochemical and spectral data(nuclear magnetic resonance,mass spectrometry and Marfey’s analyses),and the cytotoxic activities were assayed by MTT method.Results Eight metabolites,L-Ala-2-aminobenzoic acid(1),L-Ala-2-aminobenzoic acid-methyl ester(2),2S-(2-hydroxypropanamido)benzamide(3),3-(p-hydroxyphenyl)-N-methylpropionamide(4),4-hydroxycinnamamide(5),cyclo-(L-ProL-Tyr)(6),cerebroside A(7)and cerebroside B(8),were isolated and identified from the artificial seawater fermentation products.Compounds 1–8 showed weak cytotoxicities against the tested MCF-7,A549 and BV2 cells.Conclusion Eight compounds were further isolated from the fungus Penicillium chrysogenum S-3-25.Compounds 1 and 2 were new natural products and their NMR data were reported for the first time.Compounds 1–8 did not show strong cytotoxic activity.

关 键 词：海洋来源真菌 产黄青霉 人工海水培养基 代谢产物 细胞毒 

分 类 号：R931.77[医药卫生—生药学]