生物活性镁锌钙非晶薄膜及其体外降解行为  

Bioactivity and in vitro degradation behavior of MgZnCa thin-film metallic glass

作　　者：徐立明[1] 周慧慧 卞西磊 孙康[1] 付饶 刘兴旺 王刚[1] XU LiMing;ZHOU HuiHui;BIAN XiLei;SUN Kang;FU Rao;LIU XingWang;WANG Gang(Institute of Materials,Shanghai University,Shanghai 200444,China;Department of Orthopedics,Bengang General Hospital,Liaoning Healh Industry Group,Benxi 117000,China;Department of Neurology,Tongren Hospital flated to Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Department of Sports Medicine,Huashan Hospital,Fudan University,Shanghai 200040,China)

机构地区：[1]上海大学材料研究所,上海200444 [2]辽宁健康产业集团本钢总医院骨科,本溪117000 [3]上海交通大学医学院附属同仁医院神经内科,上海200025 [4]复旦大学附属华山医院运动医学科,上海200040

出　　处：《中国科学：技术科学》2022年第11期1692-1704,共13页Scientia Sinica(Technologica)

基　　金：国家自然科学基金(批准号:51925103)资助项目。

摘　　要：采用磁控溅射技术制备MgZnCa非晶合金薄膜,同时通过熔体旋淬法制备MgZnCa非晶薄带.通过电化学测试比较了MgZnCa非晶薄带和块体高纯镁在模拟体液的动电位极化曲线,发现MgZnCa非晶合金的耐腐蚀性能要优于高纯镁,这主要归因于MgZnCa非晶合金具有较高的腐蚀电位和较低的腐蚀电流密度.通过浸泡实验、析氢实验、X射线衍射测试、拉曼光谱和扫描电镜观测等方法对MgZnCa非晶合金薄膜腐蚀前后的结构、成分进行研究.结果表明,前1 h内氢气释放速率(降解)最快,并伴随着薄膜表面活泼Mg,Ca优先溶解.腐蚀膜层富锌相的出现和局部pH的增加,促进磷酸盐沉淀,进一步提高MgZnCa非晶合金耐腐蚀能力.体外细胞实验对MgZnCa非晶合金浸提液中成骨细胞的细胞活力和细胞凋亡进行检测,发现浸泡后所获得的25%浓度浸提液比对照培养基表现出更好的细胞活力和更低的细胞凋亡率,表明MgZnCa非晶合金具有良好的生物相容性.因此,优良的耐腐蚀性和生物相容性,使得MgZnCa非晶合金有潜力成为修饰生物惰性材料表面的生物活性涂层.Magnetron sputtering was used to prepare MgZnCa thin-film metallic glass(MG), and a single roller melt-spinning method was used to prepare amorphous MgZnCa ribbon. Potential polarization curves of MgZnCa amorphous ribbon and bulk high-purity magnesium(HP Mg) in simulated body fluids were compared using electrochemical tests. The corrosion resistance of MgZnCa MG is found to be significantly better than that of HP Mg, which is mainly attributed to its higher corrosion potential and lower corrosion current density. Immersion experiments, hydrogen evolution experiments, X-ray diffraction, Raman spectroscopy, and scanning electron microscopy were used to analyze the structure and composition of MgZnCa thin-film MG(TFMG) before and after corrosion,showing that the release rate of hydrogen was the fastest in the first hour, with active magnesium and calcium on the film’s surface preferentially dissolved. Furthermore, the appearance of a zinc-rich phase in the corrosion film and the increase in local pH promotes the precipitation of phosphate, which can improve the corrosion resistance of MgZnCa MG. In vitro experiments show that the 25%concentration extract obtained after soaking the MgZnCa MG has higher cell viability and lower apoptosis rate than the control medium, indicating that the MgZnCa MG is biocompatible. Therefore, with excellent corrosion resistance and biocompatibility,MgZnCa MG can be used as bioactive coatings on the surface of biologically inert materials.

关 键 词：镁锌钙非晶薄膜 腐蚀 体外细胞实验 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]