不同吸入麻醉药对新生期大鼠神经胶质细胞活化的影响  

Effects of different inhalation anesthetics on glial cells activation in neonatal rats

作　　者：张静[1] 高丹阳 于康 林晓婉 曹颖 刘晓 缪慧慧 李天佐[1] ZHANG Jing;GAO Danyang;YU Kang;LIN Xiaowan;CAO Ying;LIU Xiao;MIAO Huihui;LI Tianzuo(Department of Anesthesiology,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China)

机构地区：[1]首都医科大学附属北京世纪坛医院麻醉科,北京100038

出　　处：《中国临床药理学与治疗学》2022年第12期1391-1399,共9页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金面上项目(82271206,82071180)。

摘　　要：目的:拟观察不同吸入麻醉药对新生期大鼠神经胶质细胞增殖活化的影响。方法:新生大鼠于出生后第2天(P2)暴露于异氟醚、七氟醚或地氟醚处理2 h,并分别于出生后第7天(P7)或第14天(P14)取材,通过免疫荧光或免疫组化的方法,检测不同胶质细胞标记物(vimentin,GFAP,Iba1)在新生大鼠海马不同亚区的分布及表达。结果:大鼠在新生期(P2)单次暴露于不同吸入麻醉药,地氟醚组海马齿状回颗粒层及分子层成熟星形胶质细胞在P7及P14活化显著增强,异氟醚组仅在P14差异具有统计学意义。地氟醚组海马齿状回颗粒层而非CA1区锥体细胞层小胶质细胞在P7及P14活化显著增强,异氟醚组仅在P14存在统计学差异。结论:不同吸入麻醉药短时间单次处理出生后第2天新生大鼠,对海马不同亚区胶质细胞的活化存在不同作用,七氟醚可能对其影响最小。AIM:To examine the effects of three commonly used general anesthetics on the proliferation and activation of glial cells in neonatal rats.METHODS:Neonatal rats were exposed to either isoflurane,sevoflurane or desflurane for 2 h on postnatal day 2(P2).The animals were euthanatized and the brain were harvested on P7 and P14,respectively.The immunohistochemical localization of glial markers(vimentin,GFAP,Iba1)were examined.RESULTS:Activation of astrocyte in granular layer and molecular layer of dentate gyrus of hippocampus was significantly enhanced on P7 and P14 after desflurane exposure,while that in isoflurane group the change was only significantly different on P14.The activation of microglia in the granular layer of dentate gyrus but not in the pyramidal cell layer of CA1 region was significantly enhanced in the desflurane group on P7 and P14,while the isoflurane group only showed significant difference on P14.CONCLUSION:Short time exposure of different inhalation anesthetics has different effects on the activation of glial cells in different subregions of hippocampus in neonatal rats on postnatal day 2,and sevoflurane may have the least effect on it.

关 键 词：新生大鼠 异氟醚 七氟醚 地氟醚 星形胶质细胞 小胶质细胞 

分 类 号：R614[医药卫生—麻醉学]