基于LC-MS测定半夏泻心汤含药肠吸收液及含药血清中4种成分含量  

Content determination of four components in Banxia Xiexin Decoction medicated intestinal absorption solution and medicated serum based on LC-MS

作　　者：朱中博 魏晶晶 董俊刚 岳娟 张映红 姚雪含 霍敏峰 施丽娟 刘喜平 ZHU Zhong-bo;WEI Jing-jing;DONG Jun-gang;YUE Juan;ZHANG Ying-hong;YAO Xue-han;HUO Min-feng;SHI Li-juan;LIU Xi-ping(School of Basic Medicine,Gansu University of Chinese Medicine,Lanzhou 730000,China;Gansu Key Laboratory of Traditional Chinese Medicine Excavation,Innovation and Transformation,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学基础医学院,兰州730000 [2]甘肃省中医方药挖掘与创新转化重点实验室,兰州730000

出　　处：《中华中医药杂志》2022年第11期6714-6718,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81860813,No.81860782);甘肃省教育厅优秀研究生“创新之星”项目(No.2021CXZX-744)。

摘　　要：目的:基于液相质谱联用(LC-MS)测定中药复方半夏泻心汤大鼠含药血清及含药肠吸收液中4种特征成分含量从而建立实验用药质量标准。方法:利用LC-MS技术对半夏泻心汤大鼠含药血清及含药肠吸收液中4种特征成分进行定量分析,并进行方法学考察。结果:半夏泻心汤含药肠吸收液及含药血清中均测得黄芩苷、人参皂苷Rb1、小檗碱、甘草酸。含药肠吸收液及含药血清中含量最高成分均为黄芩苷,含量分别为(115.30±5.81)μg/m L、(52.69±1.21)ng/mL;含药肠吸收液中含量最低成分为甘草酸,含量为(5.35±0.08)ng/mL。含药血清中含量最低成分为小檗碱,含量为(1.80±0.12)ng/mL。结论:该方法可以快速、准确的检测半夏泻心汤大鼠肠吸收液、血清中特征成分甘草酸、小檗碱、人参皂苷Rb1、黄芩苷的含量差异,为后续药理实验中半夏泻心汤应用提供质量控制和理论依据。Objective:To determine four characteristic components in rat serum and intestinal absorption solution of Banxia Xiexin Decoction by liquid chromatography-mass spectrometry(LC-MS),the experimental drug quality standard was established.Methods:Four characteristic components in drug containing serum and drug containing intestinal absorption solution of Banxia Xiexin Decoction rats were quantitatively analyzed by LC-MS,and the methodology was investigated.Results:Baicalin,ginsenoside Rb1,berberine and glycyrrhizic acid were measured in the intestinal absorption solution and serum of Banxia Xiexin Decoction.Baicalin was the highest component in the drug containing intestinal absorption solution and drug containing serum,with the content of(115.30±5.81)μg/mL,(52.69±1.21)ng/mL,respectively.Glycyrrhizic acid was the lowest component in the drug containing intestinal absorption solution,with a content of(5.35±0.08)ng/mL.Berberine was the lowest component in the serum containing medicine,and the content was(1.80±0.12)ng/m L.Conclusion:This method can quickly and accurately detect the content differences of characteristic components glycyrrhizic acid,berberine,ginsenoside Rb1 and baicalin in intestinal absorption solution and serum of Banxia Xiexin Decoction rats,and provide quality control and theoretical basis for the application of Banxia Xiexin Decoction in subsequent pharmacological experiments.

关 键 词：液相质谱联用 半夏泻心汤 含药血清 含药肠吸收液 成分含量 

分 类 号：R285.5[医药卫生—中药学]