益肝消癥方联合恩替卡韦对乙型肝炎病毒诱导肝星状细胞增殖、凋亡的调节作用  

Regulatory effect of Yigan Xiaozheng Formula combined with Entecavir on proliferation and apoptosis of hepatic stellate cells induced by HBV

作　　者：刘蕊洁 曹旭[2] 杨先照[2] 梁亦骏 叶永安[2] LIU Rui-jie;CAO Xu;YANG Xian-zhao;LIANG Yi-jun;YE Yong-an(Beijing Tongren Hospital Affiliated to Capital Medical University,Beijing 100005,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]首都医科大学附属北京同仁医院,北京100005 [2]北京中医药大学东直门医院,北京100700

出　　处：《中华中医药杂志》2022年第11期6796-6800,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81603555);首都医科大学附属北京同仁医院科研基金资助课题(No.2018-YJJ-ZZL-003)。

摘　　要：目的:观察益肝消癥方联合恩替卡韦对HepG2.2.15细胞诱导的LX-2细胞增殖的抑制作用。方法:建立HepG2.2.15细胞与LX-2细胞共培养模型,CCK-8法筛选益肝消癥方含药血清最优剂量,分别以不含药血清、恩替卡韦水溶剂、益肝消癥方最优剂量联合恩替卡韦进行细胞给药,48 h后检测各组细胞HBV DNA载量、α-SMA mRNA、细胞周期及细胞凋亡水平。结果:与模型组比较,益肝消癥方+恩替卡韦组可显著降低LX-2细胞HBV DNA载量、α-SMA mRNA表达(P<0.01,P<0.05),抑制LX-2细胞增殖(P<0.05),促进LX-2细胞凋亡(P<0.05)。结论:益肝消癥方联合恩替卡韦通过抑制HepG2.2.15细胞诱导的肝星状细胞增殖、促进其凋亡,发挥抗乙肝肝硬化的作用。Objective:To observe the inhibitory effect of Yigan Xiaozheng Formula combined with Entecavir on the proliferation of LX-2 cells induced by HepG2.2.15 cells.Methods:The co-culture model of HepG2.2.15 cells and LX-2 cells was established,and the optimal dose of serum containing Yigan Xiaozheng Formula was screened by CCK-8 method.The cells were treated with serum without drug,Entecavir water solvent,and the optimal dose of Yigan Xiaozheng Formula combined with Entecavir respectively for 48 h.The HBV DNA load,α-SMA mRNA,cell cycle and apoptosis were detected.Results:Compared with the model group,Yigan Xiaozheng Formula combined with Entecavir group could reduce the HBV DNA load,α-SMA mRNA(P<0.01,P<0.05),inhibit the proliferation of LX-2 cells(P<0.05)and promote apoptosis(P<0.05).Conclusion:Yigan Xiaozheng Formula combined with Entecavir can inhibit the proliferation and apoptosis of hepatic stellate cells induced by HepG2.2.15 cells to resist hepatitis B cirrhosis.

关 键 词：HEPG2.2.15细胞 LX-2细胞 肝星状细胞 益肝消癥方 细胞增殖 细胞凋亡 机制 Α-平滑肌肌动蛋白 

分 类 号：R512.62[医药卫生—内科学] R575.2[医药卫生—临床医学]