大麦HhH-DGS全基因组鉴定及表达分析  

Genome-wide Identification of Hordeum vulgare L.HhH-DGS and Its Expression Analysis

作　　者：王容 蔡海亚[2] 焦春海[2] 安兵壮 王红盼 李博[1] 杨彩仙 徐延浩 Wang Rong;Cai Haiya;Jiao Chunhai;An Bingzhuang;Wang Hongpan;Li Bo;Yang Caixian;Xu Yanhao(College of Agriculture,Yangtze University,Jingzhou,434100;Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement,Food Crops Institute,Hubei Academy of Agricultural Sciences,Wuhan,430064)

机构地区：[1]长江大学农学院,荆州434100 [2]湖北省农业科学院粮食作物研究所,粮食作物种质创新与遗传改良湖北省重点实验室,武汉430064

出　　处：《分子植物育种》2022年第23期7657-7670,共14页Molecular Plant Breeding

基　　金：国防科工局核能开发科研项目“核辐射作物品种改良与害虫防控”和湖北省重点研发计划项目(2021BBA-225)共同资助。

摘　　要：螺旋-发夹-螺旋DNA糖基化酶超基因家族(helix-hairpin-helix DNA glycosylases superfamily,HhH-DGS)是DNA碱基切除修复(base excision repair, BER)的关键酶之一,在保持基因组的稳定中发挥着重要作用。本研究分析了大麦(Hordeum vulgare L.) HhH-DGS基因家族的分子特征、系统发育、组织表达模式并探究了该基因家族对辐射的响应规律。在大麦基因组的6条染色体上共鉴定到15个HhH-DGS基因,系统发育将其分为9个亚群。不同亚族间HhH-DGS基因外显子数量变异很大,为2~12个。种间与种内共线性分析表明,该基因家族存在于单子叶与双子叶分化前,在大麦基因组内没有发生大规模扩增。HhH-DGS基因启动子含有大量的光调节因子。大麦萌发期的茎基部及花前期的顶端组织中HhH-DGS基因表达量显著高于其他组织器官;HhH-DGS基因HORVU.MOREX.r3.7HG0667530、HORVU.MOREX.r3.3HG0221280、HORVU.MOREX.r3.3HG0223220和HORVU.MOREX.r3.6HG0561680在大麦不同的器官中稳定表达,并在4种辐射处理后表达都上调。HORVU.MOREX.r3.5HG0469860、HORVU.MOREX.r3.7HG0634400和HORVU.MOREX.r3.1HG0074440表现出特异辐射方式响应模式。本研究在解析大麦HhH-DGS基因家族的特征、进化与功能的基础上,为大麦辐射育种提供了参考。Helix-Hairpin-Helix DNA glycosylase superfamily encode a kind of key DNA glycosylase enzymes in base excision repair(BER), playing an important role in keeping genomic stability. This study analyzed the molecular features, phylogenetics, expression patterns of HhH-DGS members and explored their discipline response under radiation. The results indicated that, a total of 15 HhH-DGS genes were identified on 6 chromosomes, phylogenetic tree divided them into 9 subgroups. The number of exons in the HhH-DGS genes varied a lot in different subgroups, which were 2~12. HhH-DGS genes contained a rich of light regulators. The interspecific collinearity analysis showed that the HhH-DGS genes existed before the differentiation of monocotyledonous and dicotyledonous,and HhH-DGS members did not undergo large-scale expansion in the Hordeum vulgare L. genome. Expression levels of HhH-DGS genes in stem base during germination and apex tissue during pre-flowering were significantly higher than other organs and tissues. HhH-DGS genes HORVU.MOREX.r3.7HG0723410, HORVU.MOREX.r3.7HG0667760, HORVU.MOREX.r3.1HG0069170 and HORVU.MOREX.r3.1HG0073440 were stably expressed in different organs and up regulated under different radiation. HORVU.MOREX.r3.5HG0469860, HORVU.MOREX.r3.7HG0634400 and HORVU.MOREX.r3.1HG0074440 exhibited specific radiation response patterns.Based on the analysis of function and evolution of HhH-DGS in Hordeum vulgar L. This study provided information for mutation breeding in Hordeum vulgare L..

关 键 词：大麦 HhH-DGS家族 碱基切除修复 表达模式 辐射应答 

分 类 号：S512.3[农业科学—作物学]