水稻雌性核不育恢复系‘云岭319FS’分子标记辅助选择育种  

Marker Assisted Breeding of The Genic Female Sterile Restorer Line’Yunling319FS’ of Rice (Oryza sativa L.)

作　　者：李伟[1] 朱骞[1] 郭效琼 罗樊 吴文彬 余卫霖 李仕川 罗迪 王天杰 王镜博 陈丽娟[1,2] 李东宣[1,2] Li Wei;Zhu Qian;Guo Xiaoqiong;Luo Fan;Wu Wenbin;Yu Weilin;Li Shichuan;Luo Di;Wang Tianjie;Wang Jingbo l;Chen Lijuan;Lee Dongsun(Rice Research Institute,Yunnan Agricultural University,Kunming,65020l;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,The Key Laboratory for Crop Production and Smart Agriculture of Yunnan Province,Yunnan Agricultural University,Kunming,650201)

机构地区：[1]云南农业大学稻作研究所,昆明650201 [2]云南农业大学,云南省作物生产与智慧农业重点实验室,省部共建云南生物资源保护与利用国家重点实验室,昆明650201

出　　处：《分子植物育种》2022年第23期7826-7833,共8页Molecular Plant Breeding

基　　金：云南省重大专项(2018ZG005);国家自然科学基金项目(31860108,31560115);国家重点研发项目(2016YFD-0101101-5);2017云岭英才计划“云岭高端外国专家”项目共同资助。

摘　　要：为了获得杂交稻种子生产中理想的亲本材料,本研究以水稻雌性核不育突变体材料‘G39’为雌性核不育基因fst的供体材料,通过常规杂交选育结合水稻雌性核不育基因FST分子标记辅助选择的方法,获得了雌性败育率为100%的雌性核不育恢复系‘云岭319FS’,并获得了一种快速、准确、高效选育获得优良水稻雌性核不育恢复系的选育方法。通过对‘云岭319FS’的株高、穗长、单穗粒数、单株穗数、花粉可育率和结实率等主要农艺性状进行分析,结果显示,‘云岭319FS’植株整体表现良好,花粉可育率高,雌性败育完全且稳定,天然自交和异交结实率为0。本研究选育获得的雌性核不育恢复系‘云岭319FS’可与雄性不育系自由组配,提高水稻种质资源利用效率,具有巨大的应用前景。In order to obtain ideal parent materials for hybrid rice seed production, the rice genic female sterile mutant ’G39’ was used as the donor material of the female-sterile(fst) gene, and the genic female sterile restorer line ’Yunling319FS’ with a female abortion rate of 100% was obtained by conventional cross breeding combined with molecular marker-assisted selection(MAS) of FST gene, and here also provides a rapid, accurate and efficient breeding method for obtaining excellent rice female genic sterile restorer lines. The main agronomic traits of’Yunling319FS’, including plant height, spikelet length, total grain number per panicle, panicle number per plant,pollen fertility rate and seed-setting rate were investigated. The results showed that ’Yunling319FS’ had good agronomic characteristics, a high pollen fertility rate, complete and stable female abortion, selfing and outcrossing seed-setting rate was zero under the natural conditions. The genic female sterile restorer Line ’Yunling319FS’obtained in this study can be freely combined with male sterile lines, which can improve the utilization efficiency of rice germplasm resources and has a great application prospect.

关 键 词：水稻(Oryza sativa L.) 雌性核不育恢复系 云岭319FS 选育 

分 类 号：S511[农业科学—作物学]