大蒜农艺性状与SSR遗传多样性关联分析  

Association Analysis of Agronomic Traits and SSR Genetic Diversity in Garlic

作　　者：李菊[1] 杨亮[1] 苗明军[1] 李志[1] 王海平[2] 王培[1] 李跃建[1] 常伟[1] Li Ju;Yang Liang;Miao Mingjun;Li Zhi;Wang Haiping;Wang Pei;Li Yuejian;Chang Wei(Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest Regions(Ministry of Agriculture and Rural Affairs)Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province,Horticulture Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu,610066;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing,100081)

机构地区：[1]四川省农业科学院园艺研究所,蔬菜种质与品种创新四川省重点实验室,农业农村部西南地区园艺作物生物学与种质创制重点实验室,成都610066 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《分子植物育种》2022年第23期7857-7867,共11页Molecular Plant Breeding

基　　金：国家特色蔬菜产业技术体系成都综合试验站项目(CARS-24-G-19);四川省财政创新能力提升工程专项(2016GYSH-011);国家现代农业产业技术体系四川省蔬菜创新团队项目(川农函[2019]472号)共同资助。

摘　　要：为挖掘与大蒜重要农艺性状相关联的分子标记,本试验选用7对有多态性的SSR引物对158份大蒜种质材料进行遗传多样性分析,利用Structure 2.3.4软件分析群体遗传结构,并采用Tassel 3.0软件中一般线性模型(GLM)进行大蒜12个农艺性状与SSR标记的关联分析。结果显示,12个农艺性状差异明显,其中单头鳞茎重变异系数最大。7对SSR引物共检测到44个等位位点,平均每对引物6.286个;Shannon信息指数(I)分布范围为1.074~1.568,平均值为1.323;引物多态性信息含量(PIC)分布范围为0.563~0.749,平均值为0.640。聚类分析将该群体分为3类,从亚类群Ⅲ-1、Ⅲ-2中筛选出4份鳞茎高产的大蒜材料;群体遗传结构分析也将该群体分为3类。关联分析共检测到3个与大蒜重要农艺性状显著相关联的标记(P<0.05或P<0.01),表型变异的解释率为4.78%~25.78%,平均为13.43%。本试验筛选出的4份大蒜材料有助于鳞茎高产大蒜专用品种的选育,获得的大蒜农艺性状相关联位点可为大蒜的分子标记辅助育种提供参考。In order to discover the molecular markers which were associated with important agronomic traits of garlic, 7 pairs of polymorphic SSR primers were selected for genetic diversity analysis in 158 garlic materials.Population genetic structure was analyzed by Structure 2.3.4. Association analysis between 12 agronomic traits of garlic and SSR markers was performed by Tassel 3.0 general linear model(GLM) program. The results showed that there were significant differences among 158 garlic materials in 12 agronomic traits. Among them, the coefficient of variation of weight per bulb was the largest. A total of 44 allelic loci were detected with 7 pairs of SSR primers,with an average of 6.286 alleles per pair of SSR primers. Shannon’s information index(I) ranged from 1.074 to1.568, with an average of 1.323. Polymorphic information content(PIC) of SSR primers ranged from 0.563 to 0.749,with an average of 0.640. All accessions were divided into 3 groups by clustering analysis, and 4 garlic materials with high yield of bulb were selected from subgroups Ⅲ-1 and Ⅲ-2. Using population genetic structure analysis,the tested materials were composed of 3 groups as well. Association analysis detected 3 markers significantly associated with important agronomic traits of garlic(P<0.05 or P<0.01), explaining for 4.78%~25.78% of the phenotypic variation, with an average of 13.43%. The 4 garlic materials screened from this experiment were conducive to the breeding of garlic varieties with high bulb yield, and the allelic loci associated with agronomic traits of garlic could provide references for molecular marker-assisted breeding of garlic.

关 键 词：大蒜(Allium sativum L.) 农艺性状 SSR 遗传多样性 关联分析 

分 类 号：S633.4[农业科学—蔬菜学]