苹果溶质转运蛋白基因MdSLC35F2-like表达与花青苷积累的研究  

Study on Expression and Anthocyanin Accumulation of Solute Carrier Gene MdSLC35F2-like in Apple

作　　者：王智宇 常贝贝 刘琦 程晓帆 杜晓云 于晓丽[1] 宋来庆[1] 赵玲玲[1] WANG Zhiyu;CHANG Beibei;LIU Qi;CHENG Xiaofan;DU Xiaoyun;YU Xiaoli;SONG Laiqing;ZHAO Lingling(Key Laboratory of Apple Germplasm Innovation and Quality Control of Yantai,Yantai Academy of Agricultural Sciences,Yantai,Shandong 265500,China;College of Life Sciences,Yantai University,Yantai,Shandong 264005,China)

机构地区：[1]山东省烟台市农业科学研究院,烟台市苹果种质创新与品质调控重点实验室,山东烟台265500 [2]烟台大学生命科学学院,山东烟台264005

出　　处：《园艺学报》2022年第11期2293-2303,共11页Acta Horticulturae Sinica

基　　金：国家现代农业产业技术体系专项资金项目(CARS-28);山东省重点研发项目(2018GHZ005);山东省农业良种工程项目(2021LZGC021);山东省外专双百计划项目(WST2018013);烟台市科技发展计划项目(2020XCZX034)。

摘　　要：以‘长富2’苹果片红芽变的果皮为试材,克隆了溶质转运蛋白家族基因Md SLC35F2-like(Solute Carrier Family 35 Member F2-like)的开放阅读框(ORF),其长度为1041 bp,编码346个氨基酸。利用在线软件进行生物信息分析,该蛋白是1个稳定的疏水性α螺旋跨膜蛋白,定位于细胞膜。同源性分析表明,Md SLC35F2-like与白梨的Pb SLC35F1-like同源性最高;系统进化分析表明,其与白梨的进化亲缘关系最近,与可可、巨桉的进化亲缘关系最远。分析‘长富2号’及其片红芽变果实着色期花青苷的积累与Md SLC35F2-like表达量的变化,结果表明,摘袋后Md SLC35F2-like表达量在‘长富2号’及其2个片红芽变果实果皮中均呈先升高后降低的趋势,在摘袋后3d表达量最高,而后逐渐降低,2个芽变材料均显著高于其野生型,表达量与花青苷含量呈正相关关系。在其他颜色品种上进行验证分析,Md SLC35F2-like在红色果皮品种‘烟富3号’果皮中的表达量极显著高于绿色果皮品种‘金陵’,在红色果肉品种‘红色之爱’果肉中的表达量显著高于白肉品种‘烟农早富’。以上结果进一步证明Md SLC35F2-like与花青苷积累有关。Anthocyanin is the main pigment affecting the skin color of apple fruit.In our previous study,Md SLC35F2-like was expressed at higher levels in the‘Nagafu 2’apple red bud mutant than in the wild-type.The open reading frame(ORF)of Md SLC35F2-like was cloned,it is 1041bp,encoding 346 amino acids,which was predicted to be a hydrophobicαhelix transmembrane protein with the highest homology to the Pb SLC35F1-like of Pyrus×bretschneideri.Phylogenetic analysis showed that it had the closest evolutionary relationship with P.×bretschneideri,and the furthest evolutionary relationship with Theobroma cacao and Eucalyptus grandis.Md SLC35F2-like sequence and its expression rules before and after the fruit bagging were analyzed.After unbagging,the expression level of Md SLC35F2-like increased firstly,and then decreased with a peak on the third day.The expression level of Md SLC35F2-like in the two kinds of buds was significantly higher than that of‘Nagafu 2’.The above results indicated that the expression level of Md SLC35F2-like gene is positively correlated with anthocyanine content.Meanwhile,its expression levels in different color apple cultivars were compared.The expression of Md SLC35F2-like in red peel cultivars was significantly higher than that in green peel cultivars,and the expression level in red pulp cultivars was higher than that in non-red pulp cultivars.It suggests that Md SLC35F2-like may participate in the anthocyanine accumulation process.

关 键 词：苹果 花青苷 MdSLC35F2-like 表达分析 

分 类 号：S661.1[农业科学—果树学]