番木瓜PRSV和PLDMV复合侵染中呈中性互作  

The Neutral Interaction of Co-infection Papaya Between Papaya Ringspot Virus and Papaya Leaf Distortion Mosaic Virus

作　　者：莫翠萍 吴自林 郭泳仪 张曙光[1] 李鹏飞[1] 李华平[1] MO Cuiping;WU Zilin;GUO Yongyi;ZHANG Shuguang;LI Pengfei;LI Huaping(Guangdong Province Key Laboratory of Microbial Signals and Disease Control,College of Plant Protection,South China Agricultural University,Guangzhou 510642,China;Guangdong Sugarcane Genetic Improvement Engineering Center,Institute of Nanfan&Seed Industry,Guangdong Academy of Sciences,Guangzhou 510316,China)

机构地区：[1]华南农业大学植物病毒研究室,广东省微生物信号与作物病害重点实验室,广州510642 [2]广东省科学院南繁种业研究所,广东省甘蔗遗传改良工程中心,广州510316

出　　处：《园艺学报》2022年第11期2336-2346,共11页Acta Horticulturae Sinica

基　　金：国家重点研发计划项目(2019YFD1001800);广东省重点领域研发计划项目(2019B020217003-04)。

摘　　要：商业化的抗番木瓜环斑病毒(papayaringspotvirus,PRSV)的转基因番木瓜品种‘华农1号’的应用成功控制了华南地区该毁灭性病毒。然而近年来在广东和海南的一些转基因或非转基因植株上发现了另一种具有严重危害的病毒——番木瓜畸形花叶病毒(papayaleafdistortionmosaicvirus,PLDMV)。为了明确这两种病毒在番木瓜上的相互关系,通过人工接种分析二者在转基因和非转基因番木瓜上的侵染率、症状、细胞病理变化以及病毒积累量等特征。结果表明,‘华农1号’对PRSV有很强的抗性,但对PLDMV却高度感病,且PLDMV并不能协助PRSV克服转基因番木瓜对其的抗性;两种病毒单独侵染和复合侵染非转基因番木瓜均能引致严重的症状,二者症状无明显差异。细胞病理学分析表明,病毒侵染后叶片叶绿体萎缩且排列不紧凑,复合侵染并未对叶肉细胞造成更为严重的损害。接种PRSV的转基因番木瓜15d后PRSV积累量逐渐减少,直至检测不到;而接种PLDMV后其积累量逐渐升高并保持在一个较高的水平。在转基因和非转基因番木瓜上,PRSV的积累量始终低于PLDMV,在非转基因番木瓜上,两种病毒长期共存,并各自维持相应的病毒含量。这些结果表明,抗PRSV的转基因番木瓜‘华农1号’不抗PLDMV;PRSV和PLDMV复合侵染没有在番木瓜植株中发生协生关系,表现为中性互作。The commercialized transgenic papaya‘Huanong 1’has been utilized to successfully control the destructive virus-papaya ringspot virus(PRSV)in South China.However,another new emerging virus,papaya leaf distortion mosaic virus(PLDMV)was found in some PRSV-resistant transgenic and other non-transgenic plants in Guangdong and Hainan Provinces through a field investigation.The survey results indicated that the two viruses had co-infections on some papaya plants.To clarify the interrelationship between the two viruses on papaya,the characteristics of both virus incidence,symptoms and cellular pathology,and virus accumulation on transgenic and non-transgenic papaya were analyzed by artificial inoculation.The results showed that the transgenic papaya‘Huanong 1’was still strong resistant to PRSV,but highly susceptible to PLDMV,and PLDMV could not help PRSV overcome its resistance to transgenic papaya.In addition,it was not observed significant symptomatic difference on the transgenic and non-transgenic papaya by the single-or co-infection of two viruses.The virions of the two viruses mostly existed in different mesophyll cells,which were not more seriously damaged with co-infection of two viruses compared with single viral infection.Moreover,the accumulation of PRSV gradually decreased until it was undetectable 15 days post inoculation on the transgenic papaya,while the accumulation of PLDMV gradually increased and remained at a higher level.On transgenic or non-transgenic papaya,the accumulation of PRSV virus was lower than that of PLDMV,but the two viruses co-existed for a long time and each maintained the corresponding viral contents on the non-transgenic papaya.These results indicate that transgenic papaya‘Huanong 1’is highly susceptible to PLDMV,and PRSV and PLDMV were shown as neutral with no synergistic infected in papaya plants.

关 键 词：番木瓜 番木瓜环斑病毒 番木瓜畸形花叶病毒 复合侵染 互作分析 

分 类 号：S668.2[农业科学—果树学]