耐受性树突状细胞在胶原蛋白诱导性关节炎大鼠体内形成微嵌合体  

Tolerogenic dendritic cells form microchimerism in vivo in collagen-induced arthritis rats

作　　者：包伦敏 马贵兰 龙调玉 蒋红梅 BAO Lunmin;MA Guilan;LONG Tiaoyu;JIANG Hongmei(Department of Clinical Microbiology and Immunology,School of Clinical Laboratory Science,Guizhou Medical University,Guiyang 550025;Department of Laboratory Medicine,People's Hospital of Anshun Gity,Anshun 561099,China)

机构地区：[1]贵州医科大学医学检验学院临床微生物学及免疫学教研室,贵州贵阳550025 [2]安顺市人民医院检验科,贵州安顺561099

出　　处：《细胞与分子免疫学杂志》2022年第11期967-971,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金地区科学基金(81960393);贵州省普通本科高校自然科学研究创新群体项目(黔教合KY字[2021]016)。

摘　　要：目的 寻找耐受性树突状细胞(tDC)输注到胶原蛋白诱导关节炎(CIA)模型大鼠后形成微嵌合体的证据。方法 骨髓来源的DC前体细胞经粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素4(IL-4)及核因子κB寡核苷酸诱骗剂(NF-κB ODN decoy)诱导成tDC,再用1,1′-十八烷基-3,3,3′,3′-四甲基三碳氰基碘化物(DiR)标记后输注到CIA大鼠体内。在细胞输注的第4 h、 24 h、 5 d、 10 d、 15 d,用小动物活体成像系统观察荧光强度和分布,第15天处死大鼠,检测主要脏器荧光信号。结果 tDC高表达OX62,低表达CD80和CD86。荧光信号主要集中在胸腹部和左后关节,胸腹部荧光信号在24 h最强,左后关节荧光信号在5 d最强。在细胞输注的15 d仍能检测到荧光,并且病足左后关节始终保持较强荧光信号。离体器官肺、肝、脾、肠系膜淋巴结中荧光信号较强,肾荧光信号较弱,心脏几乎没有荧光。结论 tDC在病足关节、肺、肝、脾、肠系膜淋巴结形成微嵌合体。Objective To find evidence of microchimerism formation following infusion of tolerogenic dendritic cells(tDCs) into collagen-induced arthritis(CIA) rats. Methods Bone marrow-derived DC precursor cells were induced into tDCs by granulocyte-macrophage colony-stimulating factor(GM-CSF), interleukin 4(IL-4) and nuclear factor-κB oligonucleotide decoy(NF-κB ODN decoy) and then infused into CIA rats after labeling with 1, 1′-octadecyl-3, 3, 3′, 3′-tetramethyltricarbocyanine iodide(DiR). At 4 hours, 24 hours, 5 days, 10 days and 15 days after cell infusion, the fluorescence intensity and distribution were observed by in vivo imaging system of small animals. Rats were sacrificed on the 15th days to detect the fluorescence signals of the main organs. Results tDCs had high expression of OX62 and low expression of CD80 and CD86. The fluorescence signal was mainly concentrated in the chest, abdomen and left posterior joint, with the strongest fluorescence signal in the chest and abdomen at 24 hours and the strongest fluorescence signal in the left posterior joint at 5 days. Fluorescence could still be detected on the 15th days after cell infusion, and the left posterior joint of the diseased foot always maintained a strong fluorescence signal. In isolated organs, the fluorescence signals in the lung, liver, spleen and mesenteric lymph nodes were strong, and the kidney fluorescence signals was weak, and the heart had little fluorescence. Conclusion tDCs forms microchimerism in the diseased foot joints, lungs, liver, spleen, and mesenteric lymph nodes.

关 键 词：耐受性树突状细胞(tDC) 胶原蛋白诱导性关节炎(CIA) 微嵌合 类风湿性关节炎(RA) 

分 类 号：R392.12[医药卫生—免疫学] R457.2[医药卫生—基础医学] R684.3R593.22