新型RNA编辑系统在动物基因功能研究中的应用  

Application of novel RNA editing tools in the research of animal gene functions

作　　者：李登銮 王刚 LI Deng-Luan;WANG Gang(Precise Genome Engineering Center,School of Life Sciences,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]广州大学生命科学学院精准基因编辑工程中心,广州510006

出　　处：《生命科学》2022年第11期1442-1455,共14页Chinese Bulletin of Life Sciences

基　　金：国家自然科学基金项目(82271909)。

摘　　要：RNA编辑指遗传信息转录后对RNA核苷酸序列的改变,包括碱基替换、核苷酸的插入和删除等过程。RNA编辑系统可分为基于CRISPR-Cas13和非CRISPR-Cas13 RNA编辑系统两种,该文分别阐述了两种系统的分子机制和编辑过程,并总结了新型RNA编辑系统的优势、缺陷以及应用价值。RNA editing can be broadly defined as post-transcriptional modification of RNA nucleotide sequence,including base replacement, insertion and deletion. RNA editing systems can be divided into two kinds of RNA editing systems based on CRISPR-Cas13 included or not. This paper described the molecular mechanism and editing process of these two systems, and summarized the advantages, defects and application of the new RNA editing system.

关 键 词：RNA编辑 CRISPR-Cas13 非CRISPR-Cas13 碱基编辑 

分 类 号：Q78[生物学—分子生物学]