MALDI-TOF MS技术在传染病诊断中的应用进展  

Progress in applications of MALDI-TOF MS technology in the diagnosis of infectious diseases

作　　者：王立琴 张驰 李晓东 彭俊平[1,2] WANG LiQin;ZHANG Chi;LI XiaoDong;PENG JunPing(NHC Key Laboratory of Systems Biology of Pathogens,Institute of Pathogen Biology,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100176,China;Key Laboratory of Respiratory Disease Pathogenomics,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100176,China;Shimadzu China Innovation Center,Shimadzu(China)Co.LTD,Beijing 100020,China)

机构地区：[1]中国医学科学院&北京协和医学院病原生物学研究所,国家卫生健康委病原系统生物学重点实验室,北京100176 [2]中国医学科学院呼吸道疾病病原组研究重点实验室,北京100176 [3]岛津企业管理(中国)有限公司,北京100020

出　　处：《中国科学：生命科学》2022年第12期1858-1867,共10页Scientia Sinica(Vitae)

基　　金：中国医学科学院中央级公益性科研院所基本科研业务费(批准号:2019PT310029)资助。

摘　　要：基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry,MALDI-TOF MS)作为新兴的检测手段已被广泛应用于临床微生物实验室.相比传统基于表型的检测方法,如形态学、血清学及生化检测,MALDI-TOF MS可在短时间内对待测微生物进行物种鉴定及耐药表型分析,简化了操作流程.多项研究表明,MALDI-TOF MS在病原生物学领域具备较大的应用潜能,通过分析分子或离子的质荷比以识别或量化待测物,适用于多种类别分子的检测.依据检测靶标的不同,本文将从蛋白质与核酸两方面系统综述该技术在传染病诊断中的应用进展.Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS)has been widely used in clinical microbiology laboratories as an emerging detection method.Compared with traditional phenotypic-based detection methods such as morphology,serology,and biochemical detection,MALDI-TOF MS can identify the microorganisms and analysis phenotype of antimicrobial resistance in a short time,simplifying the operation process.A number of studies have shown that MALDI-TOF MS has great application potential in the field of pathogenic biology.It is suitable for the detection of various molecules by analyzing the mass-to-charge ratio of molecules or ions to identify or quantify the analyte.This article systematically reviews the progress in applications of MALDI-TOF MS in the diagnosis of infectious diseases from two aspects of protein and nucleic acid depending on the detection targets.

关 键 词：MALDI-TOF MS 菌种鉴定 耐药性检测 病原体检测与分型 

分 类 号：R51[医药卫生—内科学] R446[医药卫生—临床医学]