人溶菌酶基因在烟草中的表达  

Expression of a Modified Human Lysozyme Gene in Tobacco

作　　者：王冰山[1] 窦道龙[2] 张永强[1] 张猛[3] 王志兴[1] 贾士荣[1] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081 [2]中国科学院植物研究所,北京100093 [3]西北农林科技大学园艺系,杨凌712100

出　　处：《农业生物技术学报》2002年第4期338-342,共5页Journal of Agricultural Biotechnology

摘　　要：根据植物偏爱的密码子,改变G+C总量和密码子第三位碱基的G+C含量,同时避免在真核表达中影响转录、翻译及mRNA稳定性的序列如AATTAAA和ATTTA,在原氨基酸序列不变的基础上,设计并合成了适合在植物中高效表达的人溶菌酶(human lysozyme,简称HL)基因。构建了含HL基因的植物表达载体。用根癌农杆菌(Agrobacterium tumefaciens)介导法将其转化烟草(Nicotiana tabacum)。PCR和Western检测表明,HL基因在转基因烟草中得到整合和表达。提高了转基因烟草离体叶对野火(Pseudomonas syringae pv.tabaci)的抗性。A human lysozyme gene was synthesized according to the bias in plant codon usage, including the overall G+C content and the G+C content at the third position in codons of plant genes. The potential processing sites such as AATTAAA and ATTTA which may result in the reduction of transcription, translation and mRNA stability were avoided in synthetic human lysozyme gene. A plant expression vector with the human lysozyme gene was constructed and used for transformation of tobacco (Nicotiana tabacum) mediated by Agmbacterium tumefaciens. PCR and Western blotting assay indicated that the target gene was integrated into and expressed in tobacco cell. The resistance of detached leaf of transgenic plant to Pseudomonas syringae pv. tabaci was enhanced.

关 键 词：人溶菌酶基因 烟草 表达 转基因植株 抗病性 

分 类 号：S572[农业科学—烟草工业]