HPLC法测定苦柏洗剂中盐酸巴马汀、蛇床子素和盐酸小檗碱含量  

Determination of Palmatine Hydrochloride,Osthol and Berberine Hydrochloride in Kubo Lotion By HPLC

作　　者：卢向红 刘利辉 汪霞 佘林立 LU Xianghong;LIU Lihui;WANG Xia;SHE Linli(Loudi Institute for Food and Drug Inspection&Testing,Loudi 417000,China;Yueyang Xinhuada Pharmaceutical Co Ltd,Yueyang 414000,China)

机构地区：[1]湖南省娄底市食品药品检验检测所,湖南娄底417100 [2]岳阳新华达制药有限公司,湖南岳阳414000

出　　处：《中国民族民间医药》2023年第1期28-31,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立以高效液相色谱法同时测定苦柏洗剂中盐酸巴马汀、蛇床子素和盐酸小檗碱含量的方法。方法:采用高效液相色谱法,以盐酸巴马汀、蛇床子素和盐酸小檗碱作为指标成分,采用Zafex Acutfex PA-C 18(4.6 mm×250 mm,5μm)色谱柱,柱温:25℃,流动相:0.1%磷酸溶液(含15%乙腈和0.2%十二烷基硫酸钠)-乙腈(63∶37),流速:1.0 mL·min^(-1),PDA检测器,检测波长:322nm。结果:盐酸巴马汀在0.6530~9.7955μg·mL^(-1)范围内线性关系良好,r=0.9999,方法的平均回收率为97.13%,RSD为0.89%(n=6)。蛇床子素在0.3260~4.8903μg·mL^(-1)范围内线性关系良好,r=0.9998,方法的平均回收率为95.86%,RSD为0.69%(n=6)。盐酸小檗碱在16.3554~245.3304μg·mL^(-1)范围内线性关系良好,r=0.9999,方法的平均回收率为98.81%,RSD为0.80%(n=6)。结论:建立的苦柏洗剂中盐酸巴马汀、蛇床子素和盐酸小檗碱的含量测定方法,结果准确,重复性和分离度好,可用于同时测定苦柏洗剂中盐酸巴马汀、蛇床子素和盐酸小檗碱的含量,为更好地控制苦柏洗剂质量稳定和可靠提供科学依据。Objective To establish a HPLC method for the simultaneous determination of Palmatine hydrochloride、Osthol and Berberine hydrochloride in Kubo Lotion.Method With Palmatine hydrochloride、Osthol and Berberine hydrochloride as the index components,HPLC was used.Zafex Acutfex PA-C 18 column(4.6 mm×250 mm,5μm),column temperature of 25℃,a mobile phase composed of 0.1%phosphoric acid solution(containing 15%acetonitrile and 0.2%sodium dodecyl sulfate)-acetonitrile(63∶37)were adopted.Flow rate:1.00 mL·min^(-1).PDA detector,detection wavelength:322nm.Result The linear range of Palmatine hydrochloride was 0.6530~9.7955μg·mL^(-1)(r=0.9999).The average recovery of the method was 97.13%with RSD of 0.89%(n=6).The linear range of Osthol was 0.3260~4.8903μg·mL^(-1)(r=0.9998).The average recovery was 95.86%with RSD of 0.69%(n=6).The linear range of Berberine hydrochloride was 16.3554~245.3304μg·mL^(-1)(r=0.9999).The average recovery of the method was 98.81%with RSD of 0.80%(n=6).Conclusion The established method for the determination of Palmatine hydrochloride、Osthol and Berberine hydrochloride in Kubo lotion was accurate,repeatable and had good separation degree.It could be used for the simultaneous determination of Palmatine hydrochloride、Osthol and Berberine hydrochloride in Kubo lotion,and provided a scientific basis for better quality control of Kubo lotion.

关 键 词：苦柏洗剂 盐酸巴马汀 蛇床子素 盐酸小檗碱 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]