Box-Benhnken响应曲面法优化大花紫薇中科罗索酸的提取工艺  

Box-Behnken Response Surface Methodology for Optimization of Extraction Process of Corosolic Acid from Lagerstroemia speciosa

作　　者：林艾和 朱培芳 李蓉 夏宗霄 龙小妹 郭爽 范源 LIN Aihe;ZHU Peifang;LI Rong;XIA Zongxiao;LONG Xiaomei;GUO Shuang;FAN Yuan(Yunnan University of Chinese Medicine,Kunming 650500,China;Yunnan University of Chinese Medicine/Second Affiliated Hospital of Yunnan College of Traditional Chinese Medicine,Kunming 650216,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]云南中医药大学第二附属医院,云南昆明650216

出　　处：《中国民族民间医药》2023年第1期37-42,47,共7页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：云南省高校中西医结合防治甲状腺病重点实验室。

摘　　要：目的:优选大花紫薇中三萜类化合物科罗索酸的最佳提取工艺。方法:采用HPLC建立大花紫薇中CA含量测定方法,以CA含量为评价指标,通过响应曲面法优化工艺,考察乙醇体积分数、料液比、提取时间、提取次数对CA提取效果的影响。结果:CA分离度良好,在0.480~2.880μg(r=0.9999)范围内呈良好的线性关系,平均加样回收率为99.97%(RSD=0.58%)。最佳提取工艺条件为采用95%乙醇、料液比为1∶20 g/mL、回流提取60 min,提取2次。结论:本研究建立的方法结果准确、重复性好,可用于合理优化大花紫薇中CA的提取工艺。Objective To optimize the extraction process of Corosoic acid from Crape Myrcus macrophylla.Methods HPLC was used to establish a method for the determination of Ca content in Cryptophylla grandiflorum.With Ca content as the evaluation index,response surface methodology was used to optimize the process.The effects of ethanol volume fraction,liquid-solid ratio,extraction time and extraction times on the extraction efficiency of Ca were investigated.Results The Ca separation was linear in the range of 0.480-2.880μg(r=0.9999).The average recovery was 99.97%(RSD=0.58%).The optimal extraction conditions were as follows:95%ethanol,liquid to solid ratio of 1∶20 g/mL,reflux extraction for 60 min,extraction twice.Conclusion The method established in this study was accurate and reproducible,and could be used to optimize the extraction process of CA from Crape Myrrh macrophylla.

关 键 词：大花紫薇 Box-Benhnken 提取工艺 科罗索酸 

分 类 号：R284.2[医药卫生—中药学]