烟草根系烟碱代谢关键酶活性与基因表达对复合肥用量及其基肥比例的响应  

Responses of Nicotine Key Enzyme Activities and Gene Expression to Compound Fertilizer Dosage and its Proportion in Basal Fertilizer

作　　者：周俊成 郭亚利 陈发元 张恒 朱迪 彭三喜 高焕晔 ZHOU Jun-cheng;GUO Ya-li;CHEN Fa-yuan;ZHANG Heng;ZHU Di;PENG San-xi;GAO Huan-ye(College of Tobacco,Guizhou University/Key Laboratory of Tobacco Quality Research of Guizhou Province,Guiyang 550025,Guizhou China;Guizhou Tobacco Company Qianxinanzhou Company,Xingyi 562400,Guizhou China)

机构地区：[1]贵州大学烟草学院/贵州省烟草品质研究重点实验室,贵州贵阳550025 [2]贵州省烟草公司黔西南州公司,贵州兴义562400

出　　处：《亚热带植物科学》2022年第5期331-339,共9页Subtropical Plant Science

基　　金：贵州省烟草公司黔西南州公司科技计划项目(201902)。

摘　　要：以‘云烟87’(Nicotiana tabacum‘Yunyan 87’)为材料,采用田间小区试验,设置3个烟草专用复合肥用量(6.25 kg·667 m^(–2)、8.25 kg·667 m^(–2)和10.25 kg·667 m^(–2))和3个基肥比例(50%、60%和70%),探究烟草专用复合肥用量及基肥比例对其根系烟碱代谢关键酶ODC、ADC、MPO、PMT、QPRT活性及叶片基因NtODC、NtADC表达量的影响。结果表明,在烟草各生育期,不同用肥量及基肥比例对烟碱关键酶活性及基因表达的影响有所差异;旺长期前,不同复合肥用量及基肥比例对ODC、ADC、PMT、QPRT活性的影响均未达显著水平,而MPO活性随复合肥用量的增加总体呈下降趋势,随基肥比例的增大呈单峰变化。现蕾后,复合肥用量高的植株ODC和MPO活性表现较好,其中较适宜的基肥比例分别为50%、60%;ADC活性则在常规复合肥用量下较好,且对于同一复合肥用量下,又以50%基肥的活性较高;而PMT活性在常规和高复合肥用量下,以70%基肥处理较好;QPRT则表现出较大差异,QPRT活性在常规复合肥下随基肥比例的增加而降低,而高复合肥用量下则随基肥比例的增加而增强。旺长期、现蕾期的烟碱基因NtADC和NtODC表达量大致以高复合肥用量、低基肥比例处理的较高,常规施肥次之。In order to explore the effect of tobacco-specific compound fertilizer dosage and base fertilizer ratio on the activities of nicotine metabolism key enzymes in roots,i.e.ODC,ADC,MPO,PMT and QPRT,and the expression of genes NtODC and NtADC in leaves of Nicotiana tabacum plants were carried out.In this experiment,a field test was adopted,using‘Yunyan 87’as the material,and 3 levels of tobacco-specific compound fertilizer dosage(6.25 kg·667 m^(–2),8.25 kg·667 m^(–2)and 10.25 kg·667 m^(–2))and 3 basal fertilizer ratios(50%,60%and 70%).The results showed that the effects of different compound fertilizer amounts and basal fertilizer ratios on nicotine key enzyme activities and gene expression were different in each growth period;before the peak period,different compound fertilizer amounts and basal fertilizer ratios had different effects on the activities of ODC,ADC,PMT and QPRT.The significance analysis did not reach a significant level,while the activity of MPO before the peak period generally showed a downward trend with the increase of the compound fertilizer amount,and showed a single peak change with the increase of the base fertilizer ratio.After sprouting,the activities of ODC and MPO performed better at high compound fertilizer rates,and the more suitable basal fertilizer ratios were 50%and 60%,respectively;ADC activity was better with conventional compound fertilizer dosage,and for the same compound fertilizer rate,the ADC activity was higher when the base fertilizer ratio was 50%in general;while the PMT activity was better when the base fertilizer ratio was 70%under the conventional and high compound fertilizer dosages;The activity of QPRT decreased with the increase of base fertilizer proportion under conventional compound fertilizer,but increased with the increase of base fertilizer proportion under high compound fertilizer dosage.The expression levels of nicotine genes NtADC and NtODC in the prosperous and budding periods were generally higher under high compound fertilizer dosage and low bas

关 键 词：烟草专用复合肥 基肥比例 烟碱 酶活性 基因表达量 

分 类 号：S572[农业科学—烟草工业]