NP-HPLC法快速分离维生素A乙酸酯和维生素A棕榈酸酯5种顺反式异构体  

Rapid Separation of 5 Cis-trans Isomers of Vitamin A Acetate and Vitamin A Palmitate by NP-HPLC

作　　者：蒋小英 任胜科 JIANG Xiaoying;REN Shengke

机构地区：[1]浙江医药股份有限公司昌海生物分公司,浙江绍兴312300

出　　处：《上海化工》2022年第6期53-57,共5页Shanghai Chemical Industry

摘　　要：维生素A产品不同顺反式异构体生物效价差异较大,有必要建立一个能准确分离不同顺反式异构体的方法。以正相高效液相色谱(NP-HPLC)法在视黄醇的基础上建立了维生素A乙酸酯和维生素A棕榈酸酯的顺反式异构体分离及鉴定方法,能够准确分离常见的13-顺、11-顺、9-顺、9,13-顺及全反式异构体,为维生素A产品的检测和利用提供参考意见。The biological potency of different cis-trans isomers of vitamin A products varies greatly,so it is necessary to establish a method that can accurately separate different cis-trans isomers.Based on retinol,this study established a method for the separation and identification of cis-trans isomers of vitamin A acetate and vitamin A palmitate by normal phase high performance liquid chromatography(NP-HPLC),which could accurately separate common 13-cis,11-cis,9-cis,9,13-cis and all trans isomers,and provide reference for the detection and utilization of vitamin A products.

关 键 词：维生素A 顺反异构体 效价 NP-HPLC 

分 类 号：O657.73[理学—分析化学]