胎膜早破孕妇外周血和胎膜组织高迁移率族蛋白1、白细胞介素17水平及其与宫内感染的关系研究  

The expression of high mobility group protein 1 and interleukin-17 in peripheral blood and membrane tissues of pregnant women with premature rupture of membranes and its relationship with intrauterine infection

作　　者：汪洪友[1] 王永芳[1] 周建波[1] 张艳华 Wang Hongyou;Wang Yongfang;Zhou Jianbo;Zhang Yanhua(Department of Gynaecology and Obstetrics,Binhai County People′s Hospital,Yancheng 224500,China)

机构地区：[1]滨海县人民医院妇产科,盐城224500

出　　处：《中国医师进修杂志》2022年第12期1097-1101,共5页Chinese Journal of Postgraduates of Medicine

摘　　要：目的探讨胎膜早破(PROM)孕妇外周血和胎膜组织高迁移率族蛋白1(HMGB1)、白细胞介素17(IL-17)水平及其与宫内感染的关系。方法选择2019年1月至2021年6月江苏省滨海县人民医院诊治的74例胎膜早破孕妇(研究组)和同期58例健康孕妇(健康对照组),比较两组外周血、胎膜组织HMGB1、IL-17和血清CD_(8)^(+)水平;胎膜早破患者根据宫内感染情况分为绒毛膜羊膜炎组、亚临床绒毛膜羊膜炎组和正常组,比较不同感染程度的PROM孕妇外周血和胎膜组织HMGB1、IL-17和血清CD_(8)^(+)水平,分析其与宫内感染严重程度的相关性。结果研究组外周血HMGB1、胎膜组织HMGB1 mRNA、外周血IL-17、胎膜组织IL-17 mRNA及血清CD_(8)^(+)水平均高于健康对照组[(28.34±5.16)μg/L比(22.51±4.09)μg/L、0.79±0.12比0.34±0.05、(13.05±2.57)ng/L比(8.16±1.38)ng/L、0.37±0.06比0.12±0.02、0.386±0.052比0.252±0.044],差异有统计学意义(P<0.05)。PROM孕妇外周血、胎膜组织HMGB1、IL-17及血清CD_(8)^(+)水平随宫内感染程度的加重而升高(P<0.05);Spearman相关分析结果表明,PROM孕妇外周血HMGB1、胎膜组织HMGB1 mRNA和IL-17 mRNA水平与宫内感染严重程度呈正相关(r=0.336、0.316、0.311,P<0.05)。受试者工作特征曲线分析结果表明,外周血、胎膜组织HMGB1、IL-17水平及血清CD_(8)^(+)水平联合检测评估宫内感染严重程度的曲线下面积大于各指标单独检测(P<0.05)。结论PROM孕妇外周血HMGB1和胎膜组织HMGB1、IL-17水平升高,并与宫内感染严重程度呈正相关;联合检测对病情严重程度评估具有重要临床价值。Objective To investigate the expression of high mobility group protein 1(HMGB1)and interleukin-17(IL-17)in peripheral blood and membrane tissues of pregnant women with premature rupture of membranes(PROM)and its relationship with intrauterine infection.Methods Seventy-four pregnant women with PROM from January 2019 to June 2021 were selected as the study group,and 58 healthy pregnant women at the corresponding period were selected as the healthy control group.The levels of HMGB1 and IL-17 in peripheral blood and membrane tissues and serum CD_(8)^(+)were compared between the two groups.The pregnant women with PROM were divided into the chorioamnionitis group,subclinical chorioamnionitis group and normal group according to their intrauterine infection,the expression levels of HMGB1 and IL-17 in peripheral blood and membrane tissues of patients with different infection degrees were compared,and the correlation with the severity of intrauterine infection were analyzed.Results The levels of peripheral blood HMGB1,membrane tissues HMGB1,peripheral blood IL-17,membrane tissues IL-17 and serum CD_(8)^(+)in the study group were higher than those in the control group:(28.34±5.16)μg/L vs.(22.51±4.09)μg/L,0.79±0.12 vs.0.34±0.05,(13.05±2.57)ng/L vs.(8.16±1.38)ng/L,0.37±0.06 vs.0.12±0.02,0.386±0.052 vs.0.252±0.044,there were statistical differences(P<0.05).The levels of HMGB1 and IL-17 in peripheral blood and membrane tissues and serum CD_(8)^(+)were increased with the severity of severity of intrauterine infection(P<0.05).The results of Spearman correlation analysis showed that the level of peripheral blood HMGB1,membrane tissues HMGB1 and IL-17 had positively correlated with the severity of intrauterine infection(r=0.336,0.316,0.311,P<0.05).The results of receiver operating characteristic curve analysis showed that combined detection of HMGB1 and IL-17 levels in peripheral blood and membrane tissues and serum CD_(8)^(+)levels in evaluating the severity of intrauterine infection had higher area under the curve tha

关 键 词：胎膜早破 高迁移率族蛋白质类 白细胞介素17 妊娠并发症 感染性 

分 类 号：R714.433[医药卫生—妇产科学]