云南松凋落叶分解对滇重楼生理特性及药用品质的影响  

Effects of leaf litter decomposition of Pinus yunnanensis on physiological characteristics and medicinal quality in Paris polyphylla var.yunnanensis

作　　者：许凌峰 李卓蔚 郭冬琴 赵晶晶 丁博 周浓 XU Ling-feng;LI Zhuo-wei;GUO Dong-qin;ZHAO Jing-jing;DING Bo;ZHOU Nong(College of Biology and Food Engineering,The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area’s Medicinal Herbs,Municipal Development and Reform Commission,Chongqing Three Gorges University,Chongqing 404120,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆404120

出　　处：《生态学杂志》2022年第12期2361-2367,共7页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(81260622);重庆市自然科学基金项目(cstc2018jcyjAX0267);重庆市教育委员会“成渝地区双城经济圈建设”科技创新项目专项项目(KJCX2020046)资助。

摘　　要：研究云南松凋落叶分解对滇重楼生理特性及药用品质的影响,为栽培高品质的滇重楼奠定基础。采用盆栽试验,设置0、50、100、150、200 g·盆^(-1)5个凋落叶添加水平,分别于2019年6月(T1)、7月(T2)和8月(T3)进行取样。结果表明,不同剂量的云南松凋落叶对滇重楼根系活力、光合色素含量以及叶片内抗氧化酶活性的调控效果呈现明显的抑制作用,叶片内丙二醛含量则呈现明显的促进作用,同一取样时期内,随着云南松凋落叶施用量的增加其抑制或促进作用逐渐增强。云南松凋落叶降解的化感物质对滇重楼的生长发育产生了抑制作用,导致新根茎和老根茎内总皂苷含量明显下降,随着取样时期的延长,新根茎内总皂苷含量的降低幅度依次为3.68%~31.27%(T1期)、17.23%~34.69%(T2期)和10.88%~31.03%(T3期),老根茎内总皂苷含量的降低幅度依次为2.07%~25.78%(T1期)、9.47%~20.90%(T2期)和5.58%~47.81%(T3期)。可见,不同剂量的云南松凋落叶均不同程度地降低了滇重楼的药用价值。因此,云南松凋落叶能够影响滇重楼幼苗的正常生长,云南松林下“林-药复合模式”不适宜选择滇重楼。Understanding the effects of Pinus yunnanensis leaf litter decomposition on the physiological characteristics and medicinal quality would lay a foundation for the cultivation of high-quality Paris polyphylla var.yunnanensis.A pot experiment was conducted with five litter application levels,i.e.0,50,100,150,and 200 g·pot^(-1).Samplings were taken in June(T1),July(T2),and August(T3)in 2019.The results showed that different doses of P.yunnanensis leaf litter had significant inhibitory effects on root vitality,photosynthetic pigment content and antioxidant enzyme activity in Paris polyphylla var.yunnanensis,and significant promotion effects on foliar MDA content.In each sampling period,such inhibitory and promotion effects gradually increased with increasing application levels of P.yunnanensis leaf litter.The growth and development of P.polyphylla var.yunnanensis were inhibited by the allelochemicals derived from the decomposition of P.yunnanensis leaf litter,with the total saponins content in the new and old rhizomes being inhibited.With the extension of the sampling period,the total saponin contents of new rhizomes were decreased by 3.68%-31.27%(T1),17.23%-34.69%(T2),and 10.88%-31.03%(T3),and the total saponin contents of old rhizomes were decreased by 2.07%-25.78%(T1),9.47%-20.90%(T2)and 5.58%-47.81%(T3),respectively.Therefore,different amounts of P.yunnanensis leaf litter reduced the medicinal value of P.polyphylla var.yunnanensis to varying degrees.Pinus yunnanensis leaf litter can affect the growth of P.polyphylla var.yunnanensis seedlings,and thus P.polyphylla var.yunnanensis is not suitable for the forest-medicine compound model under P.yunnanensis forests.

关 键 词：化感作用 光合色素 保护酶 总皂苷 

分 类 号：S567.239[农业科学—中草药栽培]